ArticleNucleic acids research2005
Formation of linear inverted repeat amplicons following targeting of an essential gene in Leishmania.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Synthesis and Evaluation of the First Generation of Glycosylated Nucleoside Analogues as Potential Inhibitors of the Base J Metabolism in Kinetoplastid Parasites.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Knockout of protein phosphatase 1 in Leishmania major reveals its role during RNA polymerase II transcription termination.Nucleic acids research · 2023Article
- Behind Base J: The Roles of JBP1 and JBP2 on Trypanosomatids.Pathogens (Basel, Switzerland) · 2023Review
- Chromatin-Associated Protein Complexes Link DNA Base J and Transcription Termination inmSphere · 2021Article
- Genetic Interaction Between Site-Specific Epigenetic Marks and Roles of H4v in Transcription Termination inFrontiers in cell and developmental biology · 2021Article
- Article
- Epigenetic Regulation of Transcription in Trypanosomatid Protozoa.Current genomics · 2018Review
- Article
- Base J represses genes at the end of polycistronic gene clusters in Leishmania major by promoting RNAP II termination.Molecular microbiology · 2016Article
- Defining the sequence requirements for the positioning of base J in DNA using SMRT sequencing.Nucleic acids research · 2015Article
- Regulation of transcription termination by glucosylated hydroxymethyluracil, base J, in Leishmania major and Trypanosoma brucei.Nucleic acids research · 2014Article
- The epigenome of Trypanosoma brucei: a regulatory interface to an unconventional transcriptional machine.Biochimica et biophysica acta · 2014Review
- Identification of the glucosyltransferase that converts hydroxymethyluracil to base J in the trypanosomatid genome.The Journal of biological chemistry · 2014Article
- Article
- Kinetoplastid-specific histone variant functions are conserved in Leishmania major.Molecular and biochemical parasitology · 2013Article
- Glucosylated hydroxymethyluracil, DNA base J, prevents transcriptional readthrough in Leishmania.Cell · 2012Article
- JBP1 and JBP2 proteins are Fe2+/2-oxoglutarate-dependent dioxygenases regulating hydroxylation of thymidine residues in trypanosome DNA.The Journal of biological chemistry · 2012Article
- Intrachromosomal tandem duplication and repeat expansion during attempts to inactivate the subtelomeric essential gene GSH1 in Leishmania.Nucleic acids research · 2011Article
- The structural basis for recognition of base J containing DNA by a novel DNA binding domain in JBP1.Nucleic acids research · 2011Article
- Two thymidine hydroxylases differentially regulate the formation of glucosylated DNA at regions flanking polymerase II polycistronic transcription units throughout the genome of Trypanosoma brucei.Nucleic acids research · 2010Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Attempts to inactivate an essential gene in the protozoan parasite Leishmania have often led to the generation of extra copies of the wild-type alleles of the gene. In experiments with Leishmania tarentolae set up to disrupt the gene encoding the J-binding protein 1 (JBP1), a protein binding to the unusual base beta-D-glucosyl-hydroxymethyluracil (J) of Leishmania, we obtained JBP1 mutants containing linear DNA elements (amplicons) of approximately 100 kb. These amplicons consist of a long inverted repeat with telomeric repeats at both ends and contain either the two different targeting cassettes used to inactivate JBP1, or one cassette and one JBP1 gene. Each long repeat within the linear amplicons corresponds to sequences covering the JBP1 locus, starting at the telomeres upstream of JBP1 and ending in a approximately 220 bp sequence repeated in an inverted (palindromic) orientation downstream of the JBP1 locus. We propose that these amplicons have arisen by a template switch inside a DNA replication fork involving the inverted DNA repeats and helped by the gene targeting.
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