ArticleNucleic acids research2005
Modification at position 9 with 1-methyladenosine is crucial for structure and function of nematode mitochondrial tRNAs lacking the entire T-arm.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- The Complete Mitochondrial Genome ofBiology · 2026Article
- The Secret Life of NJournal of molecular biology · 2025Review
- RNA modifying enzymes shape tRNA biogenesis and function.The Journal of biological chemistry · 2024Review
- Quantification of tRNA mRNA (New York, N.Y.) · 2024Article
- Multifaceted roles of t6A biogenesis in efficiency and fidelity of mitochondrial gene expression.Nucleic acids research · 2024Article
- The tRNA identity landscape for aminoacylation and beyond.Nucleic acids research · 2023Review
- Hopeful monsters: unintended sequencing of famously malformed mite mitochondrial tRNAs reveals widespread expression and processing of sense-antisense pairs.NAR genomics and bioinformatics · 2021Article
- Naturally Occurring tRNAs With Non-canonical Structures.Frontiers in microbiology · 2020Review
- Naturally occurring dual recognition of tRNARNA biology · 2019Article
- The chemical diversity of RNA modifications.The Biochemical journal · 2019Review
- Why are Hoogsteen base pairs energetically disfavored in A-RNA compared to B-DNA?Nucleic acids research · 2018Article
- Small but large enough: structural properties of armless mitochondrial tRNAs from the nematode Romanomermis culicivorax.Nucleic acids research · 2018Article
- Article
- tRNA Modifications: Impact on Structure and Thermal Adaptation.Biomolecules · 2017Review
- Chemical and Conformational Diversity of Modified Nucleosides Affects tRNA Structure and Function.Biomolecules · 2017Review
- Transfer RNA methyltransferases with a SpoU-TrmD (SPOUT) fold and their modified nucleosides in tRNA.Biomolecules · 2017Review
- A comprehensive collection of annotations to interpret sequence variation in human mitochondrial transfer RNAs.BMC bioinformatics · 2016Article
- A WHEP Domain Regulates the Dynamic Structure and Activity of Caenorhabditis elegans Glycyl-tRNA Synthetase.The Journal of biological chemistry · 2016Article
- Recessive Mutations in TRMT10C Cause Defects in Mitochondrial RNA Processing and Multiple Respiratory Chain Deficiencies.American journal of human genetics · 2016Article
- Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides.PloS one · 2015Article
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The mitochondria of the nematode Ascaris suum have tRNAs with unusual secondary structures that lack either the T-arm or D-arm found in most other organisms. Of the twenty-two tRNA species present in the mitochondria of A.suum, twenty lack the entire T-arm and two serine tRNAs lack the D-arm. To understand how such unusual tRNAs work in the nematode mitochondrial translation system, we analyzed post-transcriptional modifications of 11 mitochondrial tRNA species purified from A.suum, 10 of which lacked a T-arm and one of which lacked a D-arm. The most characteristic feature of nematode mitochondrial tRNAs lacking a T-arm was the presence of 1-methyladenosine at position 9 (m1A9). Synthesis of T-armless tRNAs with or without the modified nucleoside showed that T-armless tRNAs without the modification had much lower aminoacylation and EF-Tu-binding activities than native tRNAs. The addition of a single methyl group to A9 of these tRNAs was sufficient to restore nearly native levels of aminoacylation and EF-Tu-binding activity as well as tertiary structure, suggesting that m1A9 is a key residue for the activity of T-armless tRNAs. Thus, m1A9 is indispensable for the structure and function of T-armless tRNAs of nematode mitochondrial origin.
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