ArticleThe Journal of cell biology1992
Effects of profilin and profilactin on actin structure and function in living cells.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 126 citations in OpenAlex.
- Myofilamental integrity of the myometrium due to cold ischaemia stress during autotransplantation in the experimental sheep model.PloS one · 2025Article
- Profilin2 regulates actin rod assembly in neuronal cells.Scientific reports · 2021Article
- Review
- Global treadmilling coordinates actin turnover and controls the size of actin networks.Nature reviews. Molecular cell biology · 2017Review
- Profilin Interaction with Actin Filament Barbed End Controls Dynamic Instability, Capping, Branching, and Motility.Developmental cell · 2016Article
- Profilin regulates F-actin network homeostasis by favoring formin over Arp2/3 complex.Developmental cell · 2015Article
- Profilin-1 serves as a gatekeeper for actin assembly by Arp2/3-dependent and -independent pathways.Developmental cell · 2015Article
- The IQGAP-related protein DGAP1 mediates signaling to the actin cytoskeleton as an effector and a sequestrator of Rac1 GTPases.Cellular and molecular life sciences : CMLS · 2014Article
- Competition and collaboration between different actin assembly pathways allows for homeostatic control of the actin cytoskeleton.Bioarchitecture · 2014Article
- Overexpression of profilin 3 affects cell elongation and F-actin organization in Arabidopsis thaliana.Plant cell reports · 2013Article
- Accelerators, Brakes, and Gears of Actin Dynamics in Dendritic Spines.The open neuroscience journal · 2009Article
- Smn depletion alters profilin II expression and leads to upregulation of the RhoA/ROCK pathway and defects in neuronal integrity.Journal of molecular neuroscience : MN · 2007Article
- Profilin inhibits pollen tube growth through actin-binding, but not poly-L-proline-binding.Planta · 2004Article
- Cytoskeleton and plant organogenesis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2002Review
- Profilin plays a role in cell elongation, cell shape maintenance, and flowering in Arabidopsis.Plant physiology · 2000Article
- Pollen profilin function depends on interaction with proline-rich motifs.The Plant cell · 1998Article
- Motile areas of leech neurites are rich in microfilaments and two actin-binding proteins: gelsolin and profilin.Proceedings. Biological sciences · 1997Article
- Characterization of maize (Zea mays) pollen profilin function in vitro and in live cells.The Biochemical journal · 1997Article
- Probing the Plant Actin Cytoskeleton during Cytokinesis and Interphase by Profilin Microinjection.The Plant cell · 1997Article
- p140mDia, a mammalian homolog of Drosophila diaphanous, is a target protein for Rho small GTPase and is a ligand for profilin.The EMBO journal · 1997Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Previous studies have yielded conflicting results concerning the physiological role of profilin, a 12-15-kD actin- and phosphoinositide-binding protein, as a regulator of actin polymerization. We have addressed this question by directly microinjecting mammalian profilins, prepared either from an E. coli expression system or from bovine brain, into living normal rat kidney (NRK) cells. The microinjection causes a dose-dependent decrease in F-actin content, as indicated by staining with fluorescent phalloidin, and a dramatic reduction of actin and alpha-actinin along stress fibers. In addition, it has a strong inhibitory effect toward the extension of lamellipodia. However, the injection of profilin causes no detectable perturbation to the cell-substrate focal contact and no apparent depolymerization of filaments in either the nonlamellipodial circumferential band or the contractile ring of dividing cells. Furthermore, cytokinesis of injected cells occurs normally as in control cells. In contrast to pure profilin, high-affinity profilin-actin complexes from brain induce an increase in total cellular F-actin content and an enhanced ruffling activity, suggesting that the complex may dissociate readily in the cell and that there may be multiple states of profilin that differ in their ability to bind or release actin molecules. Our results indicate that profilin and profilactin can function as effective regulators for at least a subset of actin filaments in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.