ArticleThe Journal of cell biology1992
Exo-endocytotic recycling of synaptic vesicles in developing processes of cultured hippocampal neurons.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 114 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
114 citing papers in PubMed, 335 citations in OpenAlex.
- Synaptic Vesicle Recycling at the Developing Presynapse.Journal of neurochemistry · 2025Review
- PHluorin-conjugated secondary nanobodies as a tool for measuring synaptic vesicle exocytosis and endocytosis.Scientific reports · 2025Article
- Glutamate Signaling and Neuroligin/Neurexin Adhesion Play Opposing Roles That Are Mediated by Major Histocompatibility Complex I Molecules in Cortical Synapse Formation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Axonal neurotransmitter release in the regulation of myelination.Bioscience reports · 2024Review
- Spastin locally amplifies microtubule dynamics to pattern the axon for presynaptic cargo delivery.Current biology : CB · 2024Article
- Protein-lipid interactions drive presynaptic assembly upstream of cell adhesion molecules.bioRxiv : the preprint server for biology · 2023Article
- Hemisynapse Formation Between Target Astrocytes and Cortical Neuron AxonsFrontiers in molecular neuroscience · 2022Article
- Temperature-dependent structural plasticity of hippocampal synapses.Frontiers in cellular neuroscience · 2022Article
- Differences in synaptic vesicle pool behavior between male and female hippocampal cultured neurons.Scientific reports · 2021Article
- The mRNA-Binding Protein RBM3 Regulates Activity Patterns and Local Synaptic Translation in Cultured Hippocampal Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Immunogold labeling of synaptic vesicle proteins in developing hippocampal neurons.Molecular brain · 2020Article
- Antibody-driven capture of synaptic vesicle proteins on the plasma membrane enables the analysis of their interactions with other synaptic proteins.Scientific reports · 2019Article
- GFP nanobodies reveal recently-exocytosed pHluorin molecules.Scientific reports · 2019Article
- Interrogating Synaptic Architecture: Approaches for Labeling Organelles and Cytoskeleton Components.Frontiers in synaptic neuroscience · 2019Review
- Postnatal Restriction of Activity-Induced CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.The EMBO journal · 2018Article
- An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins.Journal of visualized experiments : JoVE · 2018Article
- The role of spontaneous neurotransmission in synapse and circuit development.Journal of neuroscience research · 2018Review
- Article
- Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis.Nature communications · 2017Article
54 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In mature neurons synaptic vesicles (SVs) undergo cycles of exo-endocytosis at synapses. It is currently unknown whether SV exocytosis and recycling occurs also in developing axons prior to synapse formation. To address this question, we have developed an immunocytochemical assay to reveal SV exo-endocytosis in hippocampal neurons developing in culture. In this assay antibodies directed against the lumenal domain of synaptotagmin I (Syt I), an intrinsic membrane protein of SVs, are used to reveal exposure of SV membranes at the cell surface. Addition of antibodies to the culture medium of living neurons for 1 hr at 37 degrees C resulted in their rapid and specific internalization by all neuronal processes and, particularly, by axons. Double immunofluorescence and electron microscopy immunocytochemistry indicated that the antibodies were retained within SVs in cell processes and underwent cycles of exo-endocytosis in parallel with SV membranes. In contrast, another endocytotic marker, wheat germ agglutinin, was rapidly cleared from the processes and transported to the cell body. Antibody-labeled SVs were still present in axons several days after antibody loading and became clustered at presynaptic sites in parallel with synaptogenesis. These results demonstrate that SVs undergo multiple cycles of exo-endocytosis in developing neuronal processes irrespective of the presence of synaptic contacts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.