ArticleThe Journal of cell biology1992
High level expression of transfected beta- and gamma-actin genes differentially impacts on myoblast cytoarchitecture.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 126 citations in OpenAlex.
- Exploring heterogeneous expression of beta-actin (ACTB) in bladder cancer by producing a monoclonal antibody 6D6.BMC urology · 2024Article
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- The makings of the 'actin code': regulation of actin's biological function at the amino acid and nucleotide level.Journal of cell science · 2018Review
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- Interaction of microtubules with the actin cytoskeleton via cross-talk of EB1-containing +TIPs and γ-actin in epithelial cells.Oncotarget · 2016Article
- Regulation of cell proliferation by ERK and signal-dependent nuclear translocation of ERK is dependent on Tm5NM1-containing actin filaments.Molecular biology of the cell · 2015Article
- Pericyte contractility controls endothelial cell cycle progression and sprouting: insights into angiogenic switch mechanics.American journal of physiology. Cell physiology · 2014Article
- Effect of overexpression of β- and γ-actin isoforms on actin cytoskeleton organization and migration of human colon cancer cells.Histochemistry and cell biology · 2014Article
- Chromosomal localization of actin genes in the malaria mosquito Anopheles darlingi.Medical and veterinary entomology · 2013Article
- Identification of an FHL1 protein complex containing gamma-actin and non-muscle myosin IIB by analysis of protein-protein interactions.PloS one · 2013Article
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- Nonredundant roles of cytoplasmic β- and γ-actin isoforms in regulation of epithelial apical junctions.Molecular biology of the cell · 2012Article
- Proteomic analysis of colonic mucosal tissue from tuberculous and ulcerative colitis patients.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2012Article
- The actin cytoskeleton as a sensor and mediator of apoptosis.Bioarchitecture · 2012Article
- Context-dependent functional substitution of alpha-skeletal actin by gamma-cytoplasmic actin.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Article
- Barx2 controls myoblast fusion and promotes MyoD-mediated activation of the smooth muscle alpha-actin gene.The Journal of biological chemistry · 2009Article
- Inhibition of angiogenesis in vitro: a central role for beta-actin dependent cytoskeletal remodeling.Microvascular research · 2009Article
- Rotavirus spike protein VP4 binds to and remodels actin bundles of the epithelial brush border into actin bodies.Journal of virology · 2006Article
- Conditional dominant mutations in the Caenorhabditis elegans gene act-2 identify cytoplasmic and muscle roles for a redundant actin isoform.Molecular biology of the cell · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The impact of the human beta- and gamma-actin genes on myoblast cytoarchitecture was examined by their stable transfection into mouse C2 myoblasts. Transfectant C2 clones expressing high levels of human beta-actin displayed increases in cell surface area. In contrast, C2 clones with high levels of human gamma-actin expression showed decreases in cell surface area. The changes in cell morphology were accompanied by changes in actin stress-fiber organization. The beta-actin transfectants displayed well-defined filamentous organization of actin; whereas the gamma-actin transfectants displayed a more diffuse organization of the actin cables. The role of the beta-actin protein in generating the enlarged cell phenotype was examined by transfecting a mutant form of the human beta-actin gene. Transfectant cells were shown to incorporate the aberrant actin protein into stress-fiber-like structures. High level expression of the mutant beta-actin produced decreases in cell surface area and disruption of the actin microfilament network similar to that seen with transfection of the gamma-actin gene. In contrast, transfection of another mutant form of the beta-actin gene which encodes an unstable protein had no impact on cell morphology or cytoarchitecture. These results strongly suggest that it is the nature of the encoded protein that determines the morphological response of the cell. We conclude that the relative gene expression of beta- and gamma-actin is of relevance to the control of myoblast cytoarchitecture. In particular, we conclude that the beta- and gamma-actin genes encode functionally distinct cytoarchitectural information.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.