ArticleThe Journal of cell biology1992
Relationship of F-actin distribution to development of polar shape in human polymorphonuclear neutrophils.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 120 citations in OpenAlex.
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- Neutrophil diversity and function in health and disease.Signal transduction and targeted therapy · 2024Review
- Monocyte-endothelial cell interactions in vascular and tissue remodeling.Frontiers in immunology · 2023Review
- The Transmembrane Adaptor Protein LIME Is Essential for Chemokine-Mediated Migration of Effector T Cells to Inflammatiory Sites.Molecules and cells · 2020Article
- Balance between cell-substrate adhesion and myosin contraction determines the frequency of motility initiation in fish keratocytes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Alterations in dysadherin expression and F-actin reorganization: a possible mechanism of hypericin-mediated photodynamic therapy in colon adenocarcinoma cells.Cytotechnology · 2015Article
- C-kit binding properties of hesperidin (a major component of KMP6) as a potential anti-allergic agent.PloS one · 2011Article
- Amantadine inhibits platelet-activating factor induced clathrin-mediated endocytosis in human neutrophils.American journal of physiology. Cell physiology · 2009Article
- Bringing up the rear: defining the roles of the uropod.Nature reviews. Molecular cell biology · 2009Review
- Regulation of cell cytoskeleton and membrane mechanics by electric field: role of linker proteins.Biophysical journal · 2009Article
- Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility.The Journal of cell biology · 2007Article
- Simultaneous tether extraction contributes to neutrophil rolling stabilization: a model study.Biophysical journal · 2007Article
- The role of myosin II motor activity in distributing myosin asymmetrically and coupling protrusive activity to cell translocation.Molecular biology of the cell · 2006Article
- Asymmetric distribution of myosin IIB in migrating endothelial cells is regulated by a rho-dependent kinase and contributes to tail retraction.Molecular biology of the cell · 2003Article
- Motility of ActA protein-coated microspheres driven by actin polymerization.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Leukocyte polarization in cell migration and immune interactions.The EMBO journal · 1999Review
- Chemotactic peptide-induced changes of intermediate filament organization in neutrophils during granule secretion: role of cyclic guanosine monophosphate.Molecular biology of the cell · 1998Article
- Cell transit analysis of ligand-induced stiffening of polymorphonuclear leukocytes.Biophysical journal · 1998Article
- Motile areas of leech neurites are rich in microfilaments and two actin-binding proteins: gelsolin and profilin.Proceedings. Biological sciences · 1997Article
- Nerve growth factor stimulates the accumulation of beta1 integrin at the tips of filopodia in the growth cones of sympathetic neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Polymerization of actin has been associated with development of polar shape in human neutrophils (PMN). To examine the relation of filamentous actin (F-actin) distribution to shape change in PMN, we developed a method using computerized video image analysis and fluorescence microscopy to quantify distribution of F-actin in single cells. PMN were labeled with fluorescent probe NBD-phallicidin to measure filamentous actin and Texas red to assess cell thickness. We show that Texas red fluorescence is a reasonable measure of cell thickness and that correction of the NBD-phallicidin image for cell thickness using the Texas red image permits assessment of focal F-actin content. Parameters were derived that quantify total F-actin content, movement of F-actin away from the center of the cell, asymmetry of F-actin distribution, and change from round to polar shape. The sequence of change in F-actin distribution and its relation to development of polar shape in PMN was determined using these parameters. After stimulation with chemotactic peptide at 25 degrees C, F-actin polymerized first at the rim of the PMN. This was followed by development of asymmetry of F-actin distribution and change to polar shape. The dominant pseudopod developed first in the region of lower F-actin concentration followed later by polymerization of actin in the end of the developed pseudopod. Asymmetric F-actin distribution was detected in round PMN before development of polar shape. Based upon these data, asymmetric distribution of F-actin is coincident with and probably precedes development of polar shape in PMN stimulated in suspension by chemotactic peptide.
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