ArticleBiophysical journal2005
Kinetic properties of DM-nitrophen binding to calcium and magnesium.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 37 citations in OpenAlex.
- Endoplasmic Reticulum and Mitochondrial Calcium Handling Dynamically Shape Slow Afterhyperpolarizations in Vasopressin Magnocellular Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
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- Differentially poised vesicles underlie fast and slow components of release at single synapses.The Journal of general physiology · 2020Article
- Quantitative optical nanophysiology of CaNature communications · 2018Article
- Extrapolating microdomain Ca(2+) dynamics using BK channels as a Ca(2+) sensor.Scientific reports · 2016Article
- Mechanisms of beat-to-beat regulation of cardiac pacemaker cell function by Ca²⁺ cycling dynamics.Biophysical journal · 2013Article
- Readily releasable pool of synaptic vesicles measured at single synaptic contacts.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Measuring the kinetics of calcium binding proteins with flash photolysis.Biochimica et biophysica acta · 2012Review
- The contribution of RCK domains to human BK channel allosteric activation.The Journal of biological chemistry · 2012Article
- Calmodulin as a direct detector of Ca2+ signals.Nature neuroscience · 2011Article
- Alterations in Ca2+-buffering in prion-null mice: association with reduced afterhyperpolarizations in CA1 hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Resolving the fast kinetics of cooperative binding: Ca2+ buffering by calretinin.PLoS biology · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Caged-Ca(2+) compounds such as nitrophenyl-EGTA (NP-EGTA) and DM-nitrophen (DMn) are extremely useful in biological research, but their use in live cells is hampered by cytoplasmic [Mg(2+)]. We determined the properties of Ca(2+) release from NP-EGTA and DMn by using Oregon green BAPTA-5N to measure changes in [Ca(2+)] after ultraviolet flash photolysis in vitro, with or without Mg(2+) present. A large fraction (65%) of NP-EGTA, which has a negligible Mg(2+) affinity, uncages with a time constant of 10.3 ms, resulting in relatively slow increases in [Ca(2+)]. Uncaging of DMn is considerably faster, but DMn has a significant affinity for Mg(2+) to complicate the uncaging process. With experimentally determined values for the Ca(2+) and Mg(2+) binding/unbinding rates of DMn and NP-EGTA, we built a mathematical model to assess the utility of NP-EGTA and DMn in rapid Ca(2+)-uncaging experiments in the presence of Mg(2+). We discuss the advantages and disadvantages of using each compound under different conditions. To determine the kinetics of Ca(2+) binding to biologically relevant Ca(2+) buffers, such as Ca(2+)-binding proteins, the use of DMn is preferable even in the presence of Mg(2+).
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