ArticleJournal of bacteriology1992
Abnormal fractionation of beta-lactamase in Escherichia coli: evidence for an interaction with the inner membrane in the absence of a leader peptide.
Article in Journal of bacteriology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- Versatile selection technology for intracellular protein-protein interactions mediated by a unique bacterial hitchhiker transport mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Influence of N-terminal truncations on the functional expression of Bacillus licheniformis gamma-glutamyltranspeptidase in recombinant Escherichia coli.Current microbiology · 2008Article
- A bacterial two-hybrid system based on the twin-arginine transporter pathway of E. coli.Protein science : a publication of the Protein Society · 2007Article
- Genetic selection for protein solubility enabled by the folding quality control feature of the twin-arginine translocation pathway.Protein science : a publication of the Protein Society · 2006Article
- SPdb--a signal peptide database.BMC bioinformatics · 2005Article
- Membrane association of the Escherichia coli enterobactin synthase proteins EntB/G, EntE, and EntF.Journal of bacteriology · 2000Article
- Leaderless polypeptides efficiently extracted from whole cells by osmotic shock.Journal of bacteriology · 1997Article
- Targeting of signal sequenceless proteins for export in Escherichia coli with altered protein translocase.The EMBO journal · 1996Article
- Strategies for achieving high-level expression of genes in Escherichia coli.Microbiological reviews · 1996Review
- PrlA and PrlG suppressors reduce the requirement for signal sequence recognition.Journal of bacteriology · 1994Article
- A signal sequence is not required for protein export in prlA mutants of Escherichia coli.The EMBO journal · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
beta-Lactamase with the -20 to -1 region of the leader peptide deleted (almost complete deletion of the leader peptide) [delta(-20,-1) beta-lactamase] was released from Escherichia coli cells by osmotic shock. Fractionation of the cells by conversion to spheroplasts and protease accessibility experiments further indicated that a portion of the protein may be bound to the cytoplasmic membrane and be partially exposed in the periplasmic space. Expression of delta(-20,-1) beta-lactamase conferred a 25-fold increase in the 50% lethal dose for ampicillin relative to that for controls, thus confirming that a small amount (about 2%) of the active protein is completely exported from the cytoplasm. These results suggest that even in the absence of a leader peptide, mature beta-lactamase is able to interact with the cytoplasmic membrane and be translocated into the periplasmic space, albeit with a low efficiency.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.