ArticleEMBO reports2005
Nucleosome binding and histone methyltransferase activity of Drosophila PRC2.
Article in EMBO reports, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed, 173 citations in OpenAlex.
- Recurrent innovation of protein-protein interactions in the Drosophila piRNA pathway.The EMBO journal · 2026Article
- DNA glycosylases Ogg1 and Mutyh influence gene expression of PRC2 targets associated with cognition.Cellular and molecular life sciences : CMLS · 2025Article
- Review
- Genome-wide identification and characterization of polycomb repressive complex 2 core components in upland cotton (Gossypium hirsutum L.).BMC plant biology · 2023Article
- Critical Roles of Polycomb Repressive Complexes in Transcription and Cancer.International journal of molecular sciences · 2022Review
- Review
- Fusarium BP1 is a reader of H3K27 methylation.Nucleic acids research · 2021Article
- Going beyond Polycomb: EZH2 functions in prostate cancer.Oncogene · 2021Review
- The Role of Polycomb Group Protein BMI1 in DNA Repair and Genomic Stability.International journal of molecular sciences · 2021Review
- Epigenetic modifications in muscle regeneration and progression of Duchenne muscular dystrophy.Clinical epigenetics · 2021Review
- A Structural Perspective on Gene Repression by Polycomb Repressive Complex 2.Sub-cellular biochemistry · 2021Review
- Article
- Polycomb repressor complex 2 function in breast cancer (Review).International journal of oncology · 2020Review
- Post-translational modifications of PRC2: signals directing its activity.Epigenetics & chromatin · 2020Review
- Structural heterogeneity of the mammalian polycomb repressor complex in immune regulation.Experimental & molecular medicine · 2020Review
- Deregulation of Polycomb Repressive Complex-2 in Mantle Cell Lymphoma Confers Growth Advantage by Epigenetic Suppression ofFrontiers in oncology · 2020Article
- Precision Targeting with EZH2 and HDAC Inhibitors in Epigenetically Dysregulated Lymphomas.Clinical cancer research : an official journal of the American Association for Cancer Research · 2019Article
- Updating and interaction of polycomb repressive complex 2 components in maize (Zea mays).Planta · 2019Article
- Evolution and conservation of polycomb repressive complex 1 core components and putative associated factors in the green lineage.BMC genomics · 2019Article
- PRC2 recruitment and H3K27me3 deposition atScience advances · 2019Article
24 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Drosophila Polycomb group protein E(z) is a histone methyltransferase (HMTase) that is essential for maintaining HOX gene silencing during development. E(z) exists in a multiprotein complex called Polycomb repressive complex 2 (PRC2) that also contains Su(z)12, Esc and Nurf55. Reconstituted recombinant PRC2 methylates nucleosomes in vitro, but recombinant E(z) on its own shows only poor HMTase activity on nucleosomes. Here, we investigate the function of the PRC2 subunits. We show that PRC2 binds to nucleosomes in vitro but that individual PRC2 subunits alone do not bind to nucleosomes. By analysing PRC2 subcomplexes, we show that Su(z)12-Nurf55 is the minimal nucleosome-binding module of PRC2 and that Esc contributes to high-affinity binding of PRC2 nucleosomes. We find that nucleosome binding of PRC2 is not sufficient for histone methylation and that only complexes that contain Esc protein show robust HMTase activity. These observations suggest that different subunits provide mechanistically distinct functions within the PRC2 HMTase: the nucleosome-binding subunits Su(z)12 and Nurf55 anchor the E(z) enzyme on chromatin substrates, whereas Esc is needed to boost enzymatic activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.