ArticleThe EMBO journal2005
CaMKIIgamma-mediated inactivation of the Kin I kinesin MCAK is essential for bipolar spindle formation.
Article in The EMBO journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Bioinformatical analysis of the key differentially expressed genes for screening potential biomarkers in Wilms tumor.Scientific reports · 2023Article
- Multivalent Molecules as Modulators of RNA Granule Size and Composition.Biophysical journal · 2017Article
- Spatiotemporal dynamics of Aurora B-PLK1-MCAK signaling axis orchestrates kinetochore bi-orientation and faithful chromosome segregation.Scientific reports · 2015Article
- The activity regulation of the mitotic centromere-associated kinesin by Polo-like kinase 1.Oncotarget · 2015Article
- Functional analysis of phosphorylation of the mitotic centromere-associated kinesin by Aurora B kinase in human tumor cells.Cell cycle (Georgetown, Tex.) · 2015Article
- Polo-like kinase 1 regulates the stability of the mitotic centromere-associated kinesin in mitosis.Oncotarget · 2014Article
- Kv3.4 potassium channel-mediated electrosignaling controls cell cycle and survival of irradiated leukemia cells.Pflugers Archiv : European journal of physiology · 2013Article
- Microtubule plus-ends within a mitotic cell are 'moving platforms' with anchoring, signalling and force-coupling roles.Open biology · 2012Review
- Regulation of localization and activity of the microtubule depolymerase MCAK.Bioarchitecture · 2011Article
- PLK1 phosphorylates mitotic centromere-associated kinesin and promotes its depolymerase activity.The Journal of biological chemistry · 2011Article
- Functional and spatial regulation of mitotic centromere-associated kinesin by cyclin-dependent kinase 1.Molecular and cellular biology · 2010Article
- Kinesin-13s in mitosis: Key players in the spatial and temporal organization of spindle microtubules.Seminars in cell & developmental biology · 2010Review
- How kinesin motor proteins drive mitotic spindle function: Lessons from molecular assays.Seminars in cell & developmental biology · 2010Review
- Predominant regulators of tubulin monomer-polymer partitioning and their implication for cell polarization.Cellular and molecular life sciences : CMLS · 2009Review
- CaM kinase II initiates meiotic spindle depolymerization independently of APC/C activation.The Journal of cell biology · 2008Article
- MCAK-independent functions of ch-Tog/XMAP215 in microtubule plus-end dynamics.Molecular and cellular biology · 2008Article
- Article
- Mitotic centromere-associated kinesin is a novel marker for prognosis and lymph node metastasis in colorectal cancer.British journal of cancer · 2008Article
- Interphase-specific phosphorylation-mediated regulation of tubulin dimer partitioning in human cells.Molecular biology of the cell · 2007Article
- The interplay of the N- and C-terminal domains of MCAK control microtubule depolymerization activity and spindle assembly.Molecular biology of the cell · 2007Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
MCAK, a member of the kinesin-13 family, is a microtubule (MT) depolymerase that is necessary to ensure proper kinetochore MT attachment during spindle formation. Regulation of MCAK activity and localization is controlled in part by Aurora B kinase at the centromere. Here we analyzed human cells depleted of the ubiquitous Ca(2+)/calmodulin-dependent protein kinase IIgamma isoform (CaMKIIgamma) by RNA interference and found that CaMKIIgamma was necessary to suppress MCAK depolymerase activity in vivo. A functional overlap with TOGp, a MT regulator known to counteract MCAK, was suggested by similar CaMKIIgamma- and TOGp-depletion phenotypes, namely disorganized multipolar spindles. A replicating vector system, which permits inducible overexpression in cells that simultaneously synthesize interfering short hairpin RNAs, was used to dissect the functional interplay between CaMKIIgamma, TOGp, and MCAK. Our results revealed two distinct but functionally overlapping mechanisms for negative regulation of the cytosolic/centrosomal pool of MCAK. These two mechanisms, involving CaMKIIgamma and TOGp, respectively, are both essential for spindle bipolarity in a normal physiological context, but not in MCAK-depleted cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.