ArticleJournal of bacteriology2005
The presumptive magnetosome protein Mms16 is a poly(3-hydroxybutyrate) granule-bound protein (phasin) in Magnetospirillum gryphiswaldense.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 67 citations in OpenAlex.
- GdbMTB: a manually curated genomic database of magnetotactic bacteria.Database : the journal of biological databases and curation · 2026Article
- Effect of oxic and anoxic conditions on intracellular storage of polyhydroxyalkanoate and polyphosphate inFrontiers in microbiology · 2023Article
- Magnetotactic bacteria and magnetofossils: ecology, evolution and environmental implications.NPJ biofilms and microbiomes · 2022Review
- Intracellular amorphous Ca-carbonate and magnetite biomineralization by a magnetotactic bacterium affiliated to the Alphaproteobacteria.The ISME journal · 2021Article
- Development of a simple intensified fermentation strategy for growth of Magnetospirillum gryphiswaldense MSR-1: Physiological responses to changing environmental conditions.New biotechnology · 2018Article
- Polyhydroxyalkanoate-associated phasins as phylogenetically heterogeneous, multipurpose proteins.Microbial biotechnology · 2017Review
- Flow cytometry as a rapid analytical tool to determine physiological responses to changing OScientific reports · 2017Article
- Phasins, Multifaceted Polyhydroxyalkanoate Granule-Associated Proteins.Applied and environmental microbiology · 2016Review
- Comparative proteome analysis reveals four novel polyhydroxybutyrate (PHB) granule-associated proteins in Ralstonia eutropha H16.Applied and environmental microbiology · 2015Article
- A tailored galK counterselection system for efficient markerless gene deletion and chromosomal tagging in Magnetospirillum gryphiswaldense.Applied and environmental microbiology · 2014Article
- Ecology, diversity, and evolution of magnetotactic bacteria.Microbiology and molecular biology reviews : MMBR · 2013Review
- A key time point for cell growth and magnetosome synthesis of Magnetospirillum gryphiswaldense based on real-time analysis of physiological factors.Frontiers in microbiology · 2013Article
- Review
- Relevant uses of surface proteins--display on self-organized biological structures.Microbial biotechnology · 2012Review
- Deletion of a fur-like gene affects iron homeostasis and magnetosome formation in Magnetospirillum gryphiswaldense.Journal of bacteriology · 2010Article
- Cre-lox-based method for generation of large deletions within the genomic magnetosome island of Magnetospirillum gryphiswaldense.Applied and environmental microbiology · 2010Article
- In vivo display of a multisubunit enzyme complex on biogenic magnetic nanoparticles.Applied and environmental microbiology · 2009Article
- Complete genome sequence of the chemolithoautotrophic marine magnetotactic coccus strain MC-1.Applied and environmental microbiology · 2009Article
- Polyhydroxyalkanoate granules are complex subcellular organelles (carbonosomes).Journal of bacteriology · 2009Review
- Expression of green fluorescent protein fused to magnetosome proteins in microaerophilic magnetotactic bacteria.Applied and environmental microbiology · 2008Article
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Mms16 protein has been previously found to be associated with isolated magnetosomes from two Magnetospirillum strains. A function of this protein as a magnetosome-specific GTPase involved in the formation of intracellular magnetosome membrane vesicles was suggested. Here we present a study of the Mms16 protein from Magnetospirillum gryphiswaldense to clarify its function. Insertion-duplication mutagenesis of the mms16 gene did not affect the formation of magnetosome particles but resulted in the loss of the ability of M. gryphiswaldense cell extracts to activate poly(3-hydroxybutyrate) (PHB) depolymerization in vitro, which was coincident with loss of the most abundant 16-kDa polypeptide from preparations of PHB granule-bound proteins. The mms16 mutation could be functionally complemented by enhanced yellow fluorescent protein (EYFP) fused to ApdA, which is a PHB granule-bound protein (phasin) in Rhodospirillum rubrum sharing 55% identity to Mms16. Fusions of Mms16 and ApdA to enhanced green fluorescent protein (EGFP) or EYFP were colocalized in vivo with the PHB granules but not with the magnetosome particles after conjugative transfer to M. gryphiswaldense. Although the Mms16-EGFP fusion protein became detectable by Western analysis in all cell fractions upon cell disruption, it was predominantly associated with isolated PHB granules. Contrary to previous suggestions, our results argue against an essential role of Mms16 in magnetosome formation, and the previously observed magnetosome localization is likely an artifact due to unspecific adsorption during preparation. Instead, we conclude that Mms16 in vivo is a PHB granule-bound protein (phasin) and acts in vitro as an activator of PHB hydrolysis by R. rubrum PHB depolymerase PhaZ1. Accordingly, we suggest renaming the Mms16 protein of Magnetospirillum species to ApdA, as in R. rubrum.
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