ArticleJournal of bacteriology2005
Regulation of major cellulosomal endoglucanases of Clostridium thermocellum differs from that of a prominent cellulosomal xylanase.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Current models in bacterial hemicellulase-encoding gene regulation.Applied microbiology and biotechnology · 2024Review
- Transcriptomic analysis of a Clostridium thermocellum strain engineered to utilize xylose: responses to xylose versus cellobiose feeding.Scientific reports · 2020Article
- Unraveling essential cellulosomal components of the (Biotechnology for biofuels · 2019Article
- Integrated omics analyses reveal the details of metabolic adaptation ofBiotechnology for biofuels · 2017Article
- Article
- Comparison of transcriptional profiles of Clostridium thermocellum grown on cellobiose and pretreated yellow poplar using RNA-Seq.Frontiers in microbiology · 2014Article
- The emergence of Clostridium thermocellum as a high utility candidate for consolidated bioprocessing applications.Frontiers in chemistry · 2014Review
- Structure and regulation of the cellulose degradome in Clostridium cellulolyticum.Biotechnology for biofuels · 2013Article
- Proteomic analysis of Clostridium thermocellum core metabolism: relative protein expression profiles and growth phase-dependent changes in protein expression.BMC microbiology · 2012Article
- Draft genome sequences for Clostridium thermocellum wild-type strain YS and derived cellulose adhesion-defective mutant strain AD2.Journal of bacteriology · 2012Article
- Glycoside hydrolases as components of putative carbohydrate biosensor proteins in Clostridium thermocellum.Journal of industrial microbiology & biotechnology · 2011Article
- Transcriptomic analysis of Clostridium thermocellum ATCC 27405 cellulose fermentation.BMC microbiology · 2011Article
- Global gene expression patterns in Clostridium thermocellum as determined by microarray analysis of chemostat cultures on cellulose or cellobiose.Applied and environmental microbiology · 2011Article
- Clostridium thermocellum cellulosomal genes are regulated by extracytoplasmic polysaccharides via alternative sigma factors.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Demonstration of the importance for cellulose hydrolysis of CelS, the most abundant cellulosomal cellulase in Clostridium thermocellum [corrected].Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Thermobifida fusca exoglucanase Cel6B is incompatible with the cellulosomal mode in contrast to endoglucanase Cel6A.Systems and synthetic biology · 2010Article
- Article
- Transcriptional regulation of the Clostridium cellulolyticum cip-cel operon: a complex mechanism involving a catabolite-responsive element.Journal of bacteriology · 2008Article
- Global view of the Clostridium thermocellum cellulosome revealed by quantitative proteomic analysis.Journal of bacteriology · 2007Article
- Induction of the celC operon of Clostridium thermocellum by laminaribiose.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of scaffoldin-anchoring genes and one of the major processive endoglucanases (CelS) from the cellulosome of Clostridium thermocellum has been shown to be dependent on the growth rate. For the present work, we studied the gene regulation of selected cellulosomal endoglucanases and a major xylanase in order to examine the previously observed substrate-linked alterations in cellulosome composition. For this purpose, the transcript levels of genes encoding endoglucanases CelB, CelG, and CelD and the family 10 xylanase XynC were determined in batch cultures, grown on either cellobiose or cellulose, and in carbon-limited continuous cultures at different dilution rates. Under all conditions tested, the transcript levels of celB and celG were at least 10-fold higher than that of celD. Like the major processive endoglucanase CelS, the transcript levels of these endoglucanase genes were also dependent on the growth rate. Thus, at a rate of 0.04 h(-1), the levels of celB, celG, and celD were threefold higher than those obtained in cultures grown at maximal rates (0.35 h(-1)) on cellobiose. In contrast, no clear correlation was observed between the transcript level of xynC and the growth rate-the levels remained relatively high, fluctuating between 30 and 50 transcripts per cell. The results suggest that the regulation of C. thermocellum endoglucanases is similar to that of the processive endoglucanase celS but differs from that of a major cellulosomal xylanase in that expression of the latter enzyme is independent of the growth rate.
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