ArticleBlood2005
Erythrocytes are the major intravascular storage sites of nitrite in human blood.
Article in Blood, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01231165 (Comparative Randomized Single-blind Trial of Amiloride in Coronary Heart Disease), which is not on this map. Cited by 119 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Comparative Randomized Single-blind Trial of Amiloride in Coronary Heart Disease
Who cites it
119 citing papers in PubMed, 346 citations in OpenAlex.
- Measures of cellular oxidative damage following vitamin E supplementation in young patients with transfusion-dependent thalassemia: a double-blind randomized controlled trial.BMC pediatrics · 2025Trial
- Hypoxic erythrocytes mediate cardioprotection through activation of soluble guanylate cyclase and release of cyclic GMP.The Journal of clinical investigation · 2023Trial
- Trial
- Effects of Anti-TNF alpha Therapy on Blood Pressure in Resistant Hypertensive Subjects: A Randomized, Double-Blind, Placebo-Controlled Pilot Study.Arquivos brasileiros de cardiologia · 2021Trial
- Pharmacokinetics, pharmacodynamics, safety, and tolerability of nebulized sodium nitrite (AIR001) following repeat-dose inhalation in healthy subjects.Clinical pharmacokinetics · 2015Trial
- No signs of inflammation during knee surgery with ischemia: a study involving inhaled nitric oxide.Mediators of inflammation · 2014Trial
- Nitrite therapy is neuroprotective and safe in cardiac arrest survivors.Nitric oxide : biology and chemistry · 2012Trial
- Inhaled nitric oxide therapy increases blood nitrite, nitrate, and s-nitrosohemoglobin concentrations in infants with pulmonary hypertension.The Journal of pediatrics · 2012Trial
- Safety and feasibility of long-term intravenous sodium nitrite infusion in healthy volunteers.PloS one · 2011Trial
- Sodium nitrite promotes regional blood flow in patients with sickle cell disease: a phase I/II study.British journal of haematology · 2008Trial
- Sensitive and specific methodology for detection of labile NO-ferroheme complexes in vitro and in blood.Redox biology · 2026Article
- Endothelial and platelet dysfunction in systemic sclerosis with pulmonary hypertension.PloS one · 2026Article
- Reliability of biochemical assays of nitric oxide biomarkers in human skeletal muscle tissue samples.PloS one · 2026Article
- The association between vegetable-derived nitrate and nitrite intake, cardiovascular risk factors and glycemic markers in obese individuals.Scientific reports · 2025Article
- Endothelial Function Biomarkers in Hypertension.Current medicinal chemistry · 2025Review
- Hemoglobin as an oxygen gasoreceptor.Acta biochimica Polonica · 2025Review
- Erythroid anion transport, nitric oxide, and blood pressure.Frontiers in physiology · 2024Article
- Ascorbate mediates the non-enzymatic reduction of nitrite to nitric oxide.Advances in redox research · 2023Article
- Plasma eNOS Concentration in Healthy Pregnancy and in Hypertensive Disorders of Pregnancy: Evidence of Reduced Concentrations in Pre-Eclampsia from Two Independent Studies.Diseases (Basel, Switzerland) · 2023Article
- Artifacts Introduced by Sample Handling in Chemiluminescence Assays of Nitric Oxide Metabolites.Antioxidants (Basel, Switzerland) · 2023Article
59 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 2 countries.
Funding
Abstract
Plasma levels of nitrite ions have been used as an index of nitric oxide synthase (NOS) activity in vivo. Recent data suggest that nitrite is a potential intravascular repository for nitric oxide (NO), bioactivated by a nitrite reductase activity of deoxyhemoglobin. The precise levels and compartmentalization of nitrite within blood and erythrocytes have not been determined. Nitrite levels in whole blood and erythrocytes were determined using reductive chemiluminescence in conjunction with a ferricyanide-based hemoglobin oxidation assay to prevent nitrite destruction. This method yields sensitive and linear measurements of whole blood nitrite over 24 hours at room temperature. Nitrite levels measured in plasma, erythrocytes, and whole blood from 15 healthy volunteers were 121 plus or minus 9, 288 plus or minus 47, and 176 plus or minus 17 nM, indicating a surprisingly high concentration of nitrite within erythrocytes. The majority of nitrite in erythrocytes is located in the cytosol unbound to proteins. In humans, we found a significant artery-to-vein gradient of nitrite in whole blood and erythrocytes. Shear stress and acetylcholine-mediated stimulation of endothelial NOS significantly increased venous nitrite levels. These studies suggest a dynamic intravascular NO metabolism in which endothelial NOS-derived NO is stabilized as nitrite, transported by erythrocytes, and consumed during arterial-to-venous transit.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.