ArticleProceedings of the National Academy of Sciences of the United States of America2005
Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 163 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
163 citing papers in PubMed, 317 citations in OpenAlex.
- High-resolution nuclear cell biology by cryo-electron tomography.Nucleus (Austin, Tex.) · 2026Review
- Article
- High-Density Inverted Micellar Intermediates Promote Membrane Fusion of Cationic Liposomes in Drug Delivery.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Structure and organization of full-length epidermal growth factor receptor in extracellular vesicles by cryo-electron tomography.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- pytom-match-pick: A tophat-transform constraint for automated classification in template matching.Journal of structural biology: X · 2025Article
- Anti-Ebola virus mAb 3A6 protects highly viremic animals from fatal outcome via binding GPNature communications · 2025Article
- Structural biology inside multicellular specimens using electron cryotomography.Quarterly reviews of biophysics · 2025Review
- STOPGAP: an open-source package for template matching, subtomogram alignment and classification.Acta crystallographica. Section D, Structural biology · 2024Article
- The big chill: Growth ofQRB discovery · 2024Review
- Architecture and Assembly of Structurally Complex Viruses.Sub-cellular biochemistry · 2024Review
- Anti-Ebola virus mAb 3A6 with unprecedented potency protects highly viremic animals from fatal outcome and physically lifts its glycoprotein target from the virion membrane.Research square · 2023Article
- STOPGAP, an open-source package for template matching, subtomogram alignment, and classification.bioRxiv : the preprint server for biology · 2023Article
- Extensive Angular Sampling Enables the Sensitive Localization of Macromolecules in Electron Tomograms.International journal of molecular sciences · 2023Article
- Article
- Interdigitated immunoglobulin arrays form the hyperstable surface layer of the extremophilic bacteriumProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Computational methods forFrontiers in cellular and infection microbiology · 2023Review
- Cryo-electron microscopy in the fight against COVID-19-mechanism of virus entry.Frontiers in molecular biosciences · 2023Review
- Article
- ArtiaX: An electron tomography toolbox for the interactive handling of sub-tomograms in UCSF ChimeraX.Protein science : a publication of the Protein Society · 2022Article
- Membrane-assisted assembly and selective secretory autophagy of enteroviruses.Nature communications · 2022Article
103 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We used cryo-electron tomography in conjunction with single-particle averaging techniques to study the structures of frozen-hydrated envelope glycoprotein (Env) complexes on intact Moloney murine leukemia retrovirus particles. Cryo-electron tomography allows 3D imaging of viruses in toto at a resolution sufficient to locate individual macromolecules, and local averaging of abundant complexes substantially improves the resolution. The averaging of repetitive features in electron tomograms is hampered by a low signal-to-noise ratio and anisotropic resolution, which results from the "missing-wedge" effect. We developed an iterative 3D averaging algorithm that compensates for this effect and used it to determine the trimeric structure of Env to a resolution of 2.7 nm, at which individual domains can be resolved. Strikingly, the 3D reconstruction is shaped like a tripod in which the trimer penetrates the membrane at three distinct locations approximately 4.5 nm apart from one another. The Env reconstruction allows tentative docking of the x-ray crystal structure of the receptor-binding domain. This study thus provides 3D structural information regarding the prefusion conformation of an intact unstained retrovirus surface protein.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.