ArticleThe Journal of physiology2005
Spontaneously active and InsP3-activated ion channels in cell nuclei from rat cerebellar Purkinje and granule neurones.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 83 citations in OpenAlex.
- Functional Consequences of Calcium-Dependent Synapse-to-Nucleus Communication: Focus on Transcription-Dependent Metabolic Plasticity.Cold Spring Harbor perspectives in biology · 2020Review
- Properties of single-channel and whole cell ClAmerican journal of physiology. Cell physiology · 2019Article
- Inositol 1,4,5-trisphosphate receptors in the endoplasmic reticulum: A single-channel point of view.Cell calcium · 2015Review
- Monovalent cationic channel activity in the inner membrane of nuclei from skeletal muscle fibers.Biophysical journal · 2014Article
- Intracellular calcium channels: inositol-1,4,5-trisphosphate receptors.European journal of pharmacology · 2014Review
- Isolating nuclei from cultured cells for patch-clamp electrophysiology of intracellular Ca(2+) channels.Cold Spring Harbor protocols · 2013Article
- Nuclear calcium signalling in the regulation of brain function.Nature reviews. Neuroscience · 2013Review
- Patch-clamp electrophysiology of intracellular Ca2+ channels.Cold Spring Harbor protocols · 2013Article
- Calcium signaling in the liver.Comprehensive Physiology · 2013Review
- Article
- Differential regulation of the InsP₃ receptor type-1 and -2 single channel properties by InsP₃, Ca²⁺ and ATP.The Journal of physiology · 2012Article
- Neuronal activity-regulated gene transcription: how are distant synaptic signals conveyed to the nucleus?F1000Research · 2012Review
- Calcium signaling in synapse-to-nucleus communication.Cold Spring Harbor perspectives in biology · 2011Review
- Constitutive cAMP response element binding protein (CREB) activation by Alzheimer's disease presenilin-driven inositol trisphosphate receptor (InsP3R) Ca2+ signaling.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Article
- Calcium dysregulation and homeostasis of neural calcium in the molecular mechanisms of neurodegenerative diseases provide multiple targets for neuroprotection.Antioxidants & redox signaling · 2011Review
- Unitary Ca(2+) current through recombinant type 3 InsP(3) receptor channels under physiological ionic conditions.The Journal of general physiology · 2010Article
- IP(3) receptors: toward understanding their activation.Cold Spring Harbor perspectives in biology · 2010Review
- The large-conductance ion channels in the nuclear envelope of central neurons.Pflugers Archiv : European journal of physiology · 2010Article
- Visualization of inositol 1,4,5-trisphosphate receptors on the nuclear envelope outer membrane by freeze-drying and rotary shadowing for electron microscopy.Journal of structural biology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Increases in Ca(2+) concentration in the nucleus of neurones modulate gene transcription and may be involved in activity-dependent long-term plasticity, apoptosis, and neurotoxicity. Little is currently known about the regulation of Ca(2+) in the nuclei of neurones. Investigation of neuronal nuclei is hampered by the cellular heterogeneity of the brain where neurones comprise no more than 10% of the cells. The situation is further complicated by large differences in properties of different neurones. Here we report a method for isolating nuclei from identified central neurones. We employed this technique to study nuclei from rat cerebellar Purkinje and granule neurones. Patch-clamp recording from the nuclear membrane of Purkinje neurones revealed numerous large-conductance channels selective for monovalent cations. The nuclear membrane of Purkinje neurones also contained multiple InsP(3)- activated ion channels localized exclusively in the inner nuclear membrane with their receptor loci facing the nucleoplasm. In contrast, the nuclear membrane of granule neurones contained only a small number of mainly anion channels. Nuclear InsP(3) receptors (InsP(3)Rs) were activated by InsP(3) with EC(50) = 0.67 microm and a Hill coefficient of 2.5. Ca(2+) exhibited a biphasic effect on the receptors elevating its activity at low concentrations and inhibiting it at micromolar concentrations. InsP(3) in saturating concentrations did not prevent the inhibitory effect of Ca(2+), but strongly increased InsP(3)R activity at resting Ca(2+) concentrations. These data are the first evidence for the presence of intranuclear sources of Ca(2+) in neurones. Ca(2+) release from the nuclear envelope may amplify Ca(2+) transients penetrating the nucleus from the cytoplasm or generate Ca(2+) transients in the nucleus independently of the cytoplasm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.