ArticleMolecular microbiology2005
Trypanosoma brucei MOB1 is required for accurate and efficient cytokinesis but not for exit from mitosis.
Article in Molecular microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 61 citations in OpenAlex.
- In Vitro Transcriptional Response ofInternational journal of molecular sciences · 2023Article
- Article
- Cytosolic and Mitochondrial Hsp90 in Cytokinesis, Mitochondrial DNA Replication, and Drug Action in Trypanosoma brucei.Antimicrobial agents and chemotherapy · 2021Article
- MOB: Pivotal Conserved Proteins in Cytokinesis, Cell Architecture and Tissue Homeostasis.Biology · 2020Review
- Article
- Who Needs a Contractile Actomyosin Ring? The Plethora of Alternative Ways to Divide a Protozoan Parasite.Frontiers in cellular and infection microbiology · 2019Review
- Functional analyses of the CIF1-CIF2 complex in trypanosomes identify the structural motifs required for cytokinesis.Journal of cell science · 2017Article
- Article
- The essential roles of cytidine diphosphate-diacylglycerol synthase in bloodstream form Trypanosoma brucei.Molecular microbiology · 2014Article
- Regulators of Trypanosoma brucei cell cycle progression and differentiation identified using a kinome-wide RNAi screen.PLoS pathogens · 2014Article
- Distinct roles of a mitogen-activated protein kinase in cytokinesis between different life cycle forms of Trypanosoma brucei.Eukaryotic cell · 2014Article
- New insights into the molecular mechanisms of mitosis and cytokinesis in trypanosomes.International review of cell and molecular biology · 2014Review
- Potent antitrypanosomal activities of heat shock protein 90 inhibitors in vitro and in vivo.The Journal of infectious diseases · 2013Article
- Identification of a Wee1-like kinase gene essential for procyclic Trypanosoma brucei survival.PloS one · 2013Article
- Regulation of the cell division cycle in Trypanosoma brucei.Eukaryotic cell · 2012Review
- The Trypanosoma brucei AIR9-like protein is cytoskeleton-associated and is required for nucleus positioning and accurate cleavage furrow placement.Molecular microbiology · 2012Article
- Cytokinesis in bloodstream stage Trypanosoma brucei requires a family of katanins and spastin.PloS one · 2012Article
- MOB control: reviewing a conserved family of kinase regulators.Cellular signalling · 2011Review
- Phosphorylation-dependent regulation of the F-BAR protein Hof1 during cytokinesis.Genes & development · 2011Article
- The small GTPase ARL2 is required for cytokinesis in Trypanosoma brucei.Molecular and biochemical parasitology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Two MOB1 genes, MOB1-A and MOB1-B, were identified in Trypanosoma brucei. MOB1-A of T. brucei was shown to form a complex with TbPK50, a functional homologue of the Schizosaccharomyces pombe protein kinase Orb6, and immune precipitated MOB1-A exhibited histone H1 protein kinase activity. MOB1-A and TbPK50 were also shown to bind p12cks1, a cyclin-dependent kinase accessory protein. Immune fluorescence of epitope-tagged MOB1-A and MOB1-B in bloodstream form trypanosomes showed they had a punctate distribution all through the cell cytoplasm and were excluded from the nucleus throughout the cell cycle. Using RNA interference (RNAi), MOB1 was shown to be essential in both bloodstream and procyclic life cycle stages. In the bloodstream form, RNAi of MOB1 resulted, after 8 h, in a significant increase in post-mitotic cells, the majority of which had a visible cleavage furrow. This was followed by the appearance of cells with abnormal complements of nuclei and kinetoplasts, often with the number of nuclei exceeding the number of kinetoplasts. Thus, downregulation of MOB1 in the bloodstream form results in a delay in cytokinesis, and leads to a deregulation of the cell cycle, possibly through an inhibitory effect on kinetoplast replication. In contrast, downregulation of MOB1 in the procyclic form appears to impede the accuracy of cytokinesis, by allowing mispositioning of the cleavage furrow and inappropriate cytokinesis. Unlike its counterpart in budding yeast, T. brucei MOB1 does not appear to be required for mitotic exit.
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