ArticleThe Biochemical journal2005
Evidence that the cADPR signalling pathway controls calcium-mediated microneme secretion in Toxoplasma gondii.
Article in The Biochemical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 66 citations in OpenAlex.
- Phytohormones regulate asexual Toxoplasma gondii replication.Parasitology research · 2023Article
- Egress Regulatory Factors: HowPathogens (Basel, Switzerland) · 2023Review
- Regulation of phosphoinositide metabolism in Apicomplexan parasites.Frontiers in cell and developmental biology · 2023Review
- Combining IPCurrent research in microbial sciences · 2023Article
- Down the membrane hole: Ion channels in protozoan parasites.PLoS pathogens · 2022Review
- Calcium signaling in intracellular protist parasites.Current opinion in microbiology · 2021Review
- A plastid two-pore channel essential for inter-organelle communication and growth of Toxoplasma gondii.Nature communications · 2021Article
- Intracellular CaInternational journal of molecular sciences · 2021Review
- The triumvirate of signaling molecules controlling Toxoplasma microneme exocytosis: Cyclic GMP, calcium, and phosphatidic acid.PLoS pathogens · 2019Article
- Evaluation of ATM Kinase Inhibitor KU-55933 as Potential Anti-Frontiers in cellular and infection microbiology · 2019Article
- Calcium signaling and the lytic cycle of the Apicomplexan parasite Toxoplasma gondii.Biochimica et biophysica acta. Molecular cell research · 2018Review
- Pathways of host cell exit by intracellular pathogens.Microbial cell (Graz, Austria) · 2018Review
- Synthesis and Biological Evaluation of a New Structural Simplified Analogue of cADPR, a Calcium-Mobilizing Secondary Messenger Firstly Isolated from Sea Urchin Eggs.Marine drugs · 2018Article
- Molecular Signaling Involved in Entry and Exit of Malaria Parasites from Host Erythrocytes.Cold Spring Harbor perspectives in medicine · 2017Review
- InsP3 Signaling in Apicomplexan Parasites.Current topics in medicinal chemistry · 2017Review
- Optogenetic monitoring identifies phosphatidylthreonine-regulated calcium homeostasis inMicrobial cell (Graz, Austria) · 2016Article
- A Novel Polyclonal Antiserum against Toxoplasma gondii Sodium Hydrogen Exchanger 1.The Korean journal of parasitology · 2016Article
- Calcium Signaling throughout the Toxoplasma gondii Lytic Cycle: A STUDY USING GENETICALLY ENCODED CALCIUM INDICATORS.The Journal of biological chemistry · 2015Article
- The calcium signaling toolkit of the Apicomplexan parasites Toxoplasma gondii and Plasmodium spp.Cell calcium · 2015Review
- Lytic Cycle of Toxoplasma gondii: 15 Years Later.Annual review of microbiology · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The protozoan parasite Toxoplasma gondii relies on calcium-mediated exocytosis to secrete adhesins on to its surface where they can engage host cell receptors. Increases in intracellular calcium occur in response to Ins(1,4,5)P3 and caffeine, an agonist of ryanodine-responsive calcium-release channels. We examined lysates and microsomes of T. gondii and detected evidence of cADPR (cyclic ADP ribose) cyclase and hydrolase activities, the two enzymes that control the second messenger cADPR, which causes calcium release from RyR (ryanodine receptor). We also detected endogenous levels of cADPR in extracts of T. gondii. Furthermore, T. gondii microsomes that were loaded with 45Ca2+ released calcium when treated with cADPR, and the RyR antagonists 8-bromo-cADPR and Ruthenium Red blocked this response. Although T. gondii microsomes also responded to Ins(1,4,5)P3, the inhibition profiles of these calcium-release channels were mutually exclusive. The RyR antagonists 8-bromo-cADPR and dantrolene inhibited protein secretion and motility in live parasites. These results indicate that RyR calcium-release channels that respond to the second-messenger cADPR play an important role in regulating intracellular Ca2+, and hence host cell invasion, in protozoan parasites.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.