ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2005
Signaling microdomains regulate inositol 1,4,5-trisphosphate-mediated intracellular calcium transients in cultured neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Monomethyl auristatin E and paclitaxel use different mechanisms to alter intracellular calcium signaling.Biochemical pharmacology · 2025Article
- Abnormal neurovascular coupling exists in patients with peritoneal dialysis and hemodialysis: evidence from a multi-mode MRI study.Clinical kidney journal · 2025Article
- Comparison of CaCell calcium · 2017Article
- Age- and location-dependent differences in store depletion-induced h-channel plasticity in hippocampal pyramidal neurons.Journal of neurophysiology · 2014Article
- The role of chromogranin B in an animal model of multiple sclerosis.Molecular and cellular neurosciences · 2013Article
- Microdomains of muscarinic acetylcholine and Ins(1,4,5)P₃ receptors create 'Ins(1,4,5)P₃ junctions' and sites of Ca²+ wave initiation in smooth muscle.Journal of cell science · 2012Article
- Imaging adhesion and signaling dynamics in Xenopus laevis growth cones.Developmental neurobiology · 2012Review
- Understanding calcium waves and sparks in central neurons.Nature reviews. Neuroscience · 2012Review
- Article
- C-terminal domain of chromogranin B regulates intracellular calcium signaling.The Journal of biological chemistry · 2011Article
- The type I inositol 1,4,5-trisphosphate receptor interacts with protein 4.1N to mediate neurite formation through intracellular Ca waves.Neuro-Signals · 2011Article
- Article
- Inositol 1,4,5-trisphosphate receptor in chromaffin secretory granules and its relation to chromogranins.Cellular and molecular neurobiology · 2010Review
- Inositol trisphosphate 3-kinases: focus on immune and neuronal signaling.Cellular and molecular life sciences : CMLS · 2010Review
- Secretory granules in inositol 1,4,5-trisphosphate-dependent Ca2+ signaling in the cytoplasm of neuroendocrine cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Review
- Initiation and propagation of a neuronal intracellular calcium wave.Journal of computational neuroscience · 2008Article
- Chromogranin B regulates calcium signaling, nuclear factor kappaB activity, and brain natriuretic peptide production in cardiomyocytes.Circulation research · 2008Article
- Inositol 1,4,5 trisphosphate receptor and chromogranin B are concentrated in different regions of the hippocampus.Journal of neuroscience research · 2007Article
- Distribution of IP3-mediated calcium responses and their role in nuclear signalling in rat basolateral amygdala neurons.The Journal of physiology · 2007Article
- Modulation of calcium wave propagation in the dendrites and to the soma of rat hippocampal pyramidal neurons.The Journal of physiology · 2006Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Ca2+ signals in neurons use specific temporal and spatial patterns to encode unambiguous information about crucial cellular functions. To understand the molecular basis for initiation and propagation of inositol 1,4,5-trisphosphate (InsP3)-mediated intracellular Ca2+ signals, we correlated the subcellular distribution of components of the InsP3 pathway with measurements of agonist-induced intracellular Ca2+ transients in cultured rat hippocampal neurons and pheochromocytoma cells. We found specialized domains with high levels of phosphatidylinositol-4-phosphate kinase (PIPKI) and chromogranin B (CGB), proteins acting synergistically to increase InsP3 receptor (InsP3R) activity and sensitivity. In contrast, Ca2+ pumps in the plasma membrane (PMCA) and sarco-endoplasmic reticulum as well as buffers that antagonize the rise in intracellular Ca2+ were distributed uniformly. By pharmacologically blocking phosphatidylinositol-4-kinase and PIPKI or disrupting the CGB-InsP3R interaction by transfecting an interfering polypeptide fragment, we produced major changes in the initiation site and kinetics of the Ca2+ signal. This study shows that a limited number of proteins can reassemble to form unique, spatially restricted signaling domains to generate distinctive signals in different regions of the same neuron. The finding that the subcellular location of initiation sites and protein microdomains was cell type specific will help to establish differences in spatiotemporal Ca2+ signaling in different types of neurons.
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