ArticleMolecular biology of the cell2005
TIF1 Represses rDNA replication initiation, but promotes normal S phase progression and chromosome transmission in Tetrahymena.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Disruption of a ∼23-24 nucleotide small RNA pathway elevates DNA damage responses inMolecular biology of the cell · 2021Article
- An Optimized and Versatile Counter-Flow Centrifugal Elutriation Workflow to Obtain Synchronized Eukaryotic Cells.Frontiers in cell and developmental biology · 2021Article
- Checkpoint Activation of an Unconventional DNA Replication Program in Tetrahymena.PLoS genetics · 2015Article
- Developmental regulation of the Tetrahymena thermophila origin recognition complex.PLoS genetics · 2015Article
- Characterization of Leishmania donovani MCM4: expression patterns and interaction with PCNA.PloS one · 2011Article
- Tetrahymena meiotic nuclear reorganization is induced by a checkpoint kinase-dependent response to DNA damage.Molecular biology of the cell · 2009Article
- Differential targeting of Tetrahymena ORC to ribosomal DNA and non-rDNA replication origins.The EMBO journal · 2009Article
- Overexpression of mtDNA-associated AtWhy2 compromises mitochondrial function.BMC plant biology · 2008Article
- Tetrahymena ORC contains a ribosomal RNA fragment that participates in rDNA origin recognition.The EMBO journal · 2007Article
- Tetrahymena POT1a regulates telomere length and prevents activation of a cell cycle checkpoint.Molecular and cellular biology · 2007Article
- TIF1 activates the intra-S-phase checkpoint response in the diploid micronucleus and amitotic polyploid macronucleus of Tetrahymena.Molecular biology of the cell · 2006Article
- Deletion of the Tetrahymena thermophila rDNA replication fork barrier region disrupts macronuclear rDNA excision and creates a fragile site in the micronuclear genome.Nucleic acids research · 2006Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The non-ORC protein, TIF1, recognizes sequences in the Tetrahymena thermophila ribosomal DNA (rDNA) minichromosome that are required for origin activation. We show here that TIF1 represses rDNA origin firing, but is required for proper macronuclear S phase progression and division. TIF1 mutants exhibit an elongated macronuclear S phase and diminished rate of DNA replication. Despite this, replication of the rDNA minichromosome initiates precociously. Because rDNA copy number is unaffected in the polyploid macronucleus, mechanisms that prevent reinitiation appear intact. Although mutants exit macronuclear S with a wild-type DNA content, division of the amitotic macronucleus is both delayed and abnormal. Nuclear defects are also observed in the diploid mitotic micronucleus, as TIF1 mutants lose a significant fraction of their micronuclear DNA. Hence, TIF1 is required for the propagation and subsequent transmission of germline chromosomes. The broad phenotypes associated with a TIF1-deficiency suggest that this origin binding protein is required globally for the proper execution and/or monitoring of key chromosomal events during S phase and possibly at later stages of the cell cycle. We propose that micro- and macronuclear defects result from exiting the respective nuclear S phases with physically compromised chromosomes.
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