ArticleMolecular biology of the cell2005
Effects of {gamma}-tubulin complex proteins on microtubule nucleation and catastrophe in fission yeast.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
- The centriculum, a membrane reticulum that surrounds Caenorhabditis elegans centrosomes, might serve as a microtubule filter.Journal of cell science · 2026Article
- Analyses of bent spindles reveal the mechanics of anaphase B in fission yeast.Molecular biology of the cell · 2026Article
- Analyses of bent spindles reveal the mechanics of anaphase B in fission yeast.bioRxiv : the preprint server for biology · 2025Article
- MAARS Software for Automatic and Quantitative Analysis of Mitotic Progression.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Aurora B kinase erases monopolar microtubule-kinetochore arrays at the meiosis I-II transition.iScience · 2023Article
- Microtubule competition and cell growth recenter the nucleus after anaphase in fission yeast.Molecular biology of the cell · 2023Article
- Article
- The TOG protein Stu2 is regulated by acetylation.PLoS genetics · 2022Article
- Persistent growth of microtubules at low density.Molecular biology of the cell · 2021Article
- Microtubule nucleation promoters Mto1 and Mto2 regulate cytokinesis in fission yeast.Molecular biology of the cell · 2020Article
- Alp7-Mto1 and Alp14 synergize to promote interphase microtubule regrowth from the nuclear envelope.Journal of molecular cell biology · 2019Article
- Nuclear Mechanics in the Fission Yeast.Cells · 2019Review
- Effects of the microtubule nucleator Mto1 on chromosomal movement, DNA repair, and sister chromatid cohesion in fission yeast.Molecular biology of the cell · 2019Article
- Roles of end-binding 1 protein and gamma-tubulin small complex in cytokinesis and flagella formation of Giardia lamblia.MicrobiologyOpen · 2019Article
- The J-domain cochaperone Rsp1 interacts with Mto1 to organize noncentrosomal microtubule assembly.Molecular biology of the cell · 2019Article
- The XMAP215 Ortholog Alp14 Promotes Microtubule Nucleation in Fission Yeast.Current biology : CB · 2018Article
- The C-terminal region of A-kinase anchor protein 350 (AKAP350A) enables formation of microtubule-nucleation centers and interacts with pericentriolar proteins.The Journal of biological chemistry · 2017Article
- MAARS: a novel high-content acquisition software for the analysis of mitotic defects in fission yeast.Molecular biology of the cell · 2017Article
- γ-Tubulin complexes in microtubule nucleation and beyond.Molecular biology of the cell · 2015Article
- A stable microtubule array drives fission yeast polarity reestablishment upon quiescence exit.The Journal of cell biology · 2015Article
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2 authors.
Funding
Abstract
Although gamma-tubulin complexes (gamma-TuCs) are known as microtubule (MT) nucleators, their function in vivo is still poorly defined. Mto1p (also known as mbo1p or mod20p) is a gamma-TuC-associated protein that recruits gamma-TuCs specifically to cytoplasmic MT organizing centers (MTOCs) and interphase MTs. Here, we investigated gamma-TuC function by analyzing MT behavior in mto1Delta and alp4 (GCP2 homologue) mutants. These cells have free, extra-long interphase MTs that exhibit abnormal behaviors such as cycles of growth and breakage, MT sliding, treadmilling, and hyperstability. The plus ends of interphase and spindle MTs grow continuously, exhibiting catastrophe defects that are dependent on the CLIP170 tip1p. The minus ends of interphase MTs exhibit shrinkage and pauses. As mto1Delta mutants lack cytoplasmic MTOCs, cytoplasmic MTs arise from spindle or other intranuclear MTs that exit the nucleus. Our findings show that mto1p and gamma-TuCs affect multiple properties of MTs including nucleation, nuclear attachment, plus-end catastrophe, and minus-end shrinkage.
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