ArticleBMC genetics2005
X- and Y-chromosome specific variants of the amelogenin gene allow sex determination in sheep (Ovis aries) and European red deer (Cervus elaphus).
Article in BMC genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 92 citations in OpenAlex.
- Application of restriction endonuclease enzymes for sex determination in selected mammalian species.Molecular biology reports · 2025Article
- Sex Identification and Species Confirmation in Modern and Archeological Caprine Enamel.Journal of proteome research · 2025Article
- Establishing reproductive seasons for the conservation of the critically endangered Kashmir red deer Cervus Hanglu.Scientific reports · 2025Article
- A label-free quantification method for assessing sex from modern and ancient bovine tooth enamel.Scientific reports · 2024Article
- Paleoproteomics.Chemical reviews · 2022Review
- Molecular Sexing and Species Detection of Antlered European Hunting Game for Forensic Purposes.Animals : an open access journal from MDPI · 2022Article
- Reflections of ecological differences? Stress responses of sympatric Alpine chamois and red deer to weather, forage quality, and human disturbance.Ecology and evolution · 2021Article
- A multiplex PCR genotyping assay to distinguish XX and XY tissues in sheep.Molecular biology reports · 2020Article
- Novel human sex-typing strategies based on the autism candidate gene NLGN4X and its male-specific gametologue NLGN4Y.Biology of sex differences · 2019Article
- Cytogenetic Analyses in Ewes with Congenital Abnormalities of the Genital Apparatus.Animals : an open access journal from MDPI · 2019Article
- Deer antler stem cells are a novel type of cells that sustain full regeneration of a mammalian organ-deer antler.Cell death & disease · 2019Article
- Molecular sexing assays in 114 mammalian species: In silico sequence reanalysis and a unified graphical visualization of diagnostic tests.Ecology and evolution · 2019Review
- Genetic diversity and population structure of two endemicJournal of genetics · 2019Article
- Functional validation of human-specific PowerPlexBMC research notes · 2018Article
- Whole Genome Amplification of Day 3 or Day 5 Human Embryos Biopsies Provides a Suitable DNA Template for PCR-Based Techniques for Genotyping, a Complement of Preimplantation Genetic Testing.BioMed research international · 2017Article
- Development of parthenote following in vivo transfer of embryos in Capra hircus.In vitro cellular & developmental biology. Animal · 2014Article
- A quantitative real-time PCR method using an X-linked gene for sex typing in pigs.Molecular biotechnology · 2013Article
- Characteristics of X- and Y-chromosome specific regions of the amelogenin gene and a PCR-based method for sex identification in red deer (Cervus elaphus).Molecular biology reports · 2010Article
- A highly polymorphic insertion in the Y-chromosome amelogenin gene can be used for evolutionary biology, population genetics and sexing in Cetacea and Artiodactyla.BMC genetics · 2008Article
- Sex determination in 6 bovid species by duplex PCR.Journal of applied genetics · 2008Article
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2 authors.
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Abstract
backgroundSimple and precise methods for sex determination in animals are a pre-requisite for a number of applications in animal production and forensics. However, some of the existing methods depend only on the detection of Y-chromosome specific sequences. Therefore, the abscence of a signal does not necessarily mean that the sample is of female origin, because experimental errors can also lead to negative results. Thus, the detection of Y- and X-chromosome specific sequences is advantageous.
resultsA novel method for sex identification in mammals (sheep, Ovis aries and European red deer, Cervus elaphus) is described, using a polymerase chain reaction (PCR) and sequencing of a part of the amelogenin gene. A partial sequence of the amelogenin gene of sheep and red deer was obtained, which exists on both X and Y chromosomes with a deletion region on the Y chromosome. With a specific pair of primers a DNA fragment of different length between the male and female mammal was amplified.
conclusionPCR amplification using the amelogenin gene primers is useful in sex identification of samples from sheep and red deer and can be applied to DNA analysis of micro samples with small amounts of DNA such as hair roots as well as bones or embryo biopsies.
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