ArticleMolecular biotechnology2005
Reaction parameters of targeted gene repair in mammalian cells.
Article in Molecular biotechnology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Relaxed chromatin induced by histone deacetylase inhibitors improves the oligonucleotide-directed gene editing in plant cells.Journal of plant research · 2018Article
- Article
- Analyses of point mutation repair and allelic heterogeneity generated by CRISPR/Cas9 and single-stranded DNA oligonucleotides.Scientific reports · 2016Article
- Genetic spell-checking: gene editing using single-stranded DNA oligonucleotides.Plant biotechnology journal · 2016Review
- Stimulation of oligonucleotide-directed gene correction by Redβ expression and MSH2 depletion in human HT1080 cells.Molecules and cells · 2015Article
- Regulation of Gene Editing Activity Directed by Single-Stranded Oligonucleotides and CRISPR/Cas9 Systems.PloS one · 2015Article
- Proliferation of genetically modified human cells on electrospun nanofiber scaffolds.Molecular therapy. Nucleic acids · 2012Article
- Oligonucleotide delivery by nucleofection does not rescue the reduced proliferation phenotype of gene-edited cells.Nucleic acid therapeutics · 2012Article
- DNA damage response pathway and replication fork stress during oligonucleotide directed gene editing.Molecular therapy. Nucleic acids · 2012Article
- Small fragment homologous replacement: evaluation of factors influencing modification efficiency in an eukaryotic assay system.PloS one · 2012Article
- Progress and prospects: oligonucleotide-directed gene modification in mouse embryonic stem cells: a route to therapeutic application.Gene therapy · 2011Review
- DNA breakage associated with targeted gene alteration directed by DNA oligonucleotides.Mutation research · 2009Article
- Targeted correction of a thalassemia-associated beta-globin mutation induced by pseudo-complementary peptide nucleic acids.Nucleic acids research · 2009Article
- Genetic correction of splice site mutation in purified and enriched myoblasts isolated from mdx5cv mice.BMC molecular biology · 2009Article
- Progress and prospects: targeted gene alteration (TGA).Gene therapy · 2007Review
- Article
- Manipulation of cell cycle progression can counteract the apparent loss of correction frequency following oligonucleotide-directed gene repair.BMC molecular biology · 2007Article
- Delivery and mechanistic considerations for the production of knock-in mice by single-stranded oligonucleotide gene targeting.Gene therapy · 2007Article
- Reduction of gene repair by selenomethionine with the use of single-stranded oligonucleotides.BMC molecular biology · 2007Article
- Gene therapy progress and prospects: targeted gene repair.Gene therapy · 2005Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Targeted gene repair uses short DNA oligonucleotides to direct a nucleotide exchange reaction at a designated site in a mammalian chromosome. The widespread use of this technique has been hampered by the inability of workers to achieve robust levels of correction. Here, we present a mammalian cell system in which DLD-1 cells bearing integrated copies of a mutant eGFP gene are repaired by modified single-stranded DNA oligonucleotides. We demonstrate that two independent clonal isolates, which are transcribed at different levels, are corrected at different frequencies. We confirm the evidence of a strand bias observed previously in other systems, wherein an oligonucleotide designed to be complementary to the nontranscribed strand of the target directs a higher level of repair than one targeting the transcribed strand. Higher concentrations of cell oligonucleotides in the electroporation mixture lead to higher levels of correction. When the target cell population is synchronized into S phase then released before electroporation, the correction efficiency is increased within the entire population. This model system could be useful for pharmacogenomic applications of targeted gene repair including the creation of cell lines containing single-base alterations.
Indexed as
Identifiers
15767697What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.