ArticleMolecular and cellular biology2005
Potential autoregulation of transcription factor PU.1 by an upstream regulatory element.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
103 citing papers in PubMed, 173 citations in OpenAlex.
- Review
- Interaction between nuclear-translocated cellular communication network factor 2 and purine-rich box 1 regulates the expression of fibrosis-related genes.Journal of cell communication and signaling · 2025Article
- Super-enhancer-associated long noncoding RNA lnc-SPI1U mediates SPI1 feedback regulation by interacting with HNRNPH1 and HNRNPF.Oncogene · 2025Article
- The epigenetic circle: feedback loops in the maintenance of cellular memory.Epigenetics & chromatin · 2025Review
- Decreased PU.1 expression in mature B cells induces lymphomagenesis.Cancer science · 2024Article
- Chromatin structure and 3D architecture define the differential functions of PU.1 regulatory elements in blood cell lineages.Epigenetics & chromatin · 2024Article
- Effects of SPI1-mediated transcriptome remodeling on Alzheimer's disease-related phenotypes in mouse models of Aβ amyloidosis.Nature communications · 2024Article
- Unraveling the enigma: housekeeping geneFrontiers in psychiatry · 2024Article
- From Genotype to Phenotype: How Enhancers Control Gene Expression and Cell Identity in Hematopoiesis.HemaSphere · 2023Review
- A lncRNA identifieseLife · 2023Article
- Blood stem cell PU.1 upregulation is a consequence of differentiation without fast autoregulation.The Journal of experimental medicine · 2022Article
- The complex landscape of haematopoietic lineage commitments is encoded in the coarse-grained endogenous network.Royal Society open science · 2021Article
- A stochastic model of homeostasis: The roles of noise and nuclear positioning in deciding cell fate.iScience · 2021Article
- Mutational synergy during leukemia induction remodels chromatin accessibility, histone modifications and three-dimensional DNA topology to alter gene expression.Nature genetics · 2021Article
- Stage-specific action of Runx1 and GATA3 controls silencing of PU.1 expression in mouse pro-T cells.The Journal of experimental medicine · 2021Article
- Hydroxycarbamide effects on DNA methylation and gene expression in myeloproliferative neoplasms.Genome research · 2021Article
- Review
- Modest changes in Spi1 dosage reveal the potential for altered microglial function as seen in Alzheimer's disease.Scientific reports · 2021Article
- VECTOR: An Integrated Correlation Network Database for the Identification of CeRNA Axes in Uveal Melanoma.Genes · 2021Article
- CircSPI1 acts as an oncogene in acute myeloid leukemia through antagonizing SPI1 and interacting with microRNAs.Cell death & disease · 2021Article
43 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 4 institutions in 4 countries.
Funding
Abstract
Regulation of the hematopoietic transcription factor PU.1 (Spi-1) plays a critical role in the development of white cells, and abnormal expression of PU.1 can lead to leukemia. We previously reported that the PU.1 promoter cannot induce expression of a reporter gene in vivo, and cell-type-specific expression of PU.1 in stable lines was conferred by a 3.4-kb DNA fragment including a DNase I hypersensitive site located 14 kb upstream of the transcription start site. Here we demonstrate that this kb -14 site confers lineage-specific reporter gene expression in vivo. This kb -14 upstream regulatory element contains two 300-bp regions which are highly conserved in five mammalian species. In Friend virus-induced erythroleukemia, the spleen focus-forming virus integrates into the PU.1 locus between these two conserved regions. DNA binding experiments demonstrated that PU.1 itself and Elf-1 bind to a highly conserved site within the proximal homologous region in vivo. A mutation of this site abolishing binding of PU.1 and Elf-1 led to a marked decrease in the ability of this upstream element to direct activity of reporter gene in myelomonocytic cell lines. These data suggest that a potential positive autoregulatory loop mediated through an upstream regulatory element is essential for proper PU.1 gene expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.