ArticleMolecular and cellular biology2005
Promoter occupancy is a major determinant of chromatin remodeling enzyme requirements.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 52 citations in OpenAlex.
- Article
- Nucleosome Remodeling at the YeastInternational journal of molecular sciences · 2023Article
- Partitioned usage of chromatin remodelers by nucleosome-displacing factors.Cell reports · 2022Article
- Article
- Capturing and Understanding the Dynamics and Heterogeneity of Gene Expression in the Living Cell.International journal of molecular sciences · 2020Review
- SWI/SNF recruitment to a DNA double-strand break by the NuA4 and Gcn5 histone acetyltransferases.DNA repair · 2015Article
- The RSC chromatin remodeling complex has a crucial role in the complete remodeler set for yeast PHO5 promoter opening.Nucleic acids research · 2014Article
- Compositional and structural analysis of selected chromosomal domains from Saccharomyces cerevisiae.Nucleic acids research · 2014Article
- The yeast PHO5 promoter: from single locus to systems biology of a paradigm for gene regulation through chromatin.Nucleic acids research · 2014Review
- Article
- Chromatin and transcription in yeast.Genetics · 2012Review
- Histone H3 lysine 4 hypermethylation prevents aberrant nucleosome remodeling at the PHO5 promoter.Molecular and cellular biology · 2011Article
- Article
- In vitro reconstitution of PHO5 promoter chromatin remodeling points to a role for activator-nucleosome competition in vivo.Molecular and cellular biology · 2010Article
- SWI/SNF has intrinsic nucleosome disassembly activity that is dependent on adjacent nucleosomes.Molecular cell · 2010Article
- Chromatin regulation and gene centrality are essential for controlling fitness pleiotropy in yeast.PloS one · 2009Article
- The yeast RNA polymerase II-associated factor Iwr1p is involved in the basal and regulated transcription of specific genes.The Journal of biological chemistry · 2009Article
- Coupling phosphate homeostasis to cell cycle-specific transcription: mitotic activation of Saccharomyces cerevisiae PHO5 by Mcm1 and Forkhead proteins.Molecular and cellular biology · 2009Article
- Differential cofactor requirements for histone eviction from two nucleosomes at the yeast PHO84 promoter are determined by intrinsic nucleosome stability.Molecular and cellular biology · 2009Article
- Role of chromatin states in transcriptional memory.Biochimica et biophysica acta · 2009Review
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Chromatin creates transcriptional barriers that are overcome by coactivator activities such as histone acetylation by Gcn5 and ATP-dependent chromatin remodeling by SWI/SNF. Factors defining the differential coactivator requirements in the transactivation of various promoters remain elusive. Induction of the Saccharomyces cerevisiae PHO5 promoter does not require Gcn5 or SWI/SNF under fully inducing conditions of no phosphate. We show that PHO5 activation is highly dependent on both coactivators at intermediate phosphate concentrations, conditions that reduce the nuclear concentration of the Pho4 transactivator and severely diminish its association with PHO5 in the absence of Gcn5 or SWI/SNF. Conversely, physiological increases in Pho4 nuclear concentration and binding at PHO5 suppress the need for both Gcn5 and SWI/SNF, suggesting that coactivator redundancy is established at high Pho4 binding site occupancy. Consistent with this, we demonstrate, using chromatin immunoprecipitation, that Gcn5 and SWI/SNF are directly recruited to PHO5 and other strongly transcribed promoters, including GAL1-10, RPL19B, RPS22B, PYK1, and EFT2, which do not require either coactivator for expression. These results show that activator concentration and binding site occupancy play crucial roles in defining the extent to which transcription requires individual chromatin remodeling enzymes. In addition, Gcn5 and SWI/SNF associate with many more genomic targets than previously appreciated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.