ArticleMolecular and cellular biology2005
Evidence that the elongation factor TFIIS plays a role in transcription initiation at GAL1 in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 53 citations in OpenAlex.
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- Muscleblind-like proteins dimerize by forming disulfide bonds to regulate alternative splicing and pathogenic RNA foci formation.bioRxiv : the preprint server for biology · 2026Article
- The general transcription factors (GTFs) of RNA polymerase II and their roles in plant development and stress responses.Critical reviews in biochemistry and molecular biology · 2024Review
- Structural basis of a transcription pre-initiation complex on a divergent promoter.Molecular cell · 2023Article
- Elongin functions as a loading factor for Mediator at ATF6α-regulated ER stress response genes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Elongation Factor TFIIS Prevents Transcription Stress and R-Loop Accumulation to Maintain Genome Stability.Molecular cell · 2019Article
- Systematic Gene-to-Phenotype Arrays: A High-Throughput Technique for Molecular Phenotyping.Molecular cell · 2018Article
- Subtracting the sequence bias from partially digested MNase-seq data reveals a general contribution of TFIIS to nucleosome positioning.Epigenetics & chromatin · 2017Article
- The transcript cleavage factor paralogue TFS4 is a potent RNA polymerase inhibitor.Nature communications · 2017Article
- Multisubunit DNA-Dependent RNA Polymerases from Vaccinia Virus and Other Nucleocytoplasmic Large-DNA Viruses: Impressions from the Age of Structure.Microbiology and molecular biology reviews : MMBR · 2017Review
- Chromatin potentiates transcription.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Transcription factors TFIIF and TFIIS promote transcript elongation by RNA polymerase II by synergistic and independent mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- PHD and TFIIS-Like domains of the Bye1 transcription factor determine its multivalent genomic distribution.PloS one · 2014Article
- Architecture of an RNA polymerase II transcription pre-initiation complex.Science (New York, N.Y.) · 2013Article
- TFIIS is required for the balanced expression of the genes encoding ribosomal components under transcriptional stress.Nucleic acids research · 2012Article
- Transcription factor IIS cooperates with the E3 ligase UBR5 to ubiquitinate the CDK9 subunit of the positive transcription elongation factor B.The Journal of biological chemistry · 2011Article
- The structure of an Iws1/Spt6 complex reveals an interaction domain conserved in TFIIS, Elongin A and Med26.The EMBO journal · 2010Article
- Phosphorylation of the transcription elongation factor Spt5 by yeast Bur1 kinase stimulates recruitment of the PAF complex.Molecular and cellular biology · 2009Article
- The basal initiation machinery: beyond the general transcription factors.Current opinion in cell biology · 2009Review
- Tiny RNAs associated with transcription start sites in animals.Nature genetics · 2009Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
TFIIS is a transcription elongation factor that has been extensively studied biochemically. Although the in vitro mechanisms by which TFIIS stimulates RNA transcript cleavage and polymerase read-through have been well characterized, its in vivo roles remain unclear. To better understand TFIIS function in vivo, we have examined its role during Gal4-mediated activation of the Saccharomyces cerevisiae GAL1 gene. Surprisingly, TFIIS is strongly associated with the GAL1 upstream activating sequence. In addition, TFIIS recruitment to Gal4-binding sites is dependent on Gal4, SAGA, and Mediator but not on RNA polymerase II (Pol II). The association of TFIIS is also necessary for the optimal recruitment of TATA-binding protein and Pol II to the GAL1 promoter. These results provide strong evidence that TFIIS plays an important role in the initiation of transcription at GAL1 in addition to its well-characterized roles in transcription elongation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.