ArticleMolecular and cellular biology2005
Brh2-Dss1 interplay enables properly controlled recombination in Ustilago maydis.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 64 citations in OpenAlex.
- Moss BRCA2 lacking the canonical DNA-binding domain promotes homologous recombination and binds to DNA.Nucleic acids research · 2025Article
- CRISPR/Cas9-Targeted Disruption of Two Highly HomologousInternational journal of molecular sciences · 2023Article
- The Pathogenic R3052W BRCA2 Variant Disrupts Homology-Directed Repair by Failing to Localize to the Nucleus.Frontiers in genetics · 2022Article
- Yeast as a Model to Unravel New BRCA2 Functions in Cell Metabolism.Frontiers in oncology · 2022Article
- Role of BRCA2 DNA-binding and C-terminal domain in its mobility and conformation in DNA repair.eLife · 2021Article
- BRCA1 and BRCA2 Tumor Suppressor Function in Meiosis.Frontiers in cell and developmental biology · 2021Review
- DSS1 and ssDNA regulate oligomerization of BRCA2.Nucleic acids research · 2020Article
- Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis.DNA repair · 2018Article
- Dss1 Regulates Association of Brh2 with Rad51.Biochemistry · 2017Article
- Article
- Homologous recombination and human health: the roles of BRCA1, BRCA2, and associated proteins.Cold Spring Harbor perspectives in biology · 2015Review
- Dual DNA-binding domains shape the interaction of Brh2 with DNA.DNA repair · 2014Article
- Quality control of homologous recombination.Cellular and molecular life sciences : CMLS · 2014Review
- Review
- DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells.Protein & cell · 2014Article
- A cancer-associated BRCA2 mutation reveals masked nuclear export signals controlling localization.Nature structural & molecular biology · 2013Article
- Brh2 and Rad51 promote telomere maintenance in Ustilago maydis, a new model system of DNA repair proteins at telomeres.DNA repair · 2013Article
- Trypanosoma brucei BRCA2 acts in a life cycle-specific genome stability process and dictates BRC repeat number-dependent RAD51 subnuclear dynamics.Nucleic acids research · 2013Article
- Dss1 release activates DNA binding potential in Brh2.Biochemistry · 2012Article
- Brh2 domain function distinguished by differential cellular responses to DNA damage and replication stress.Molecular microbiology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Brh2, the BRCA2 homolog in Ustilago maydis, functions in recombinational repair of DNA damage by regulating Rad51 and is, in turn, regulated by Dss1. Dss1 is not required for Brh2 stability in vivo, nor for Brh2 to associate with Rad51, but is required for formation of green fluorescent protein (GFP)-Rad51 foci following DNA damage by gamma radiation. To understand more about the interplay between Brh2 and Dss1, we isolated mutant variants of Brh2 able to bypass the requirement for Dss1. These variants were found to lack the entire C-terminal DNA-Dss1 binding domain but to maintain the N-terminal region harboring the Rad51-interacting BRC element. GFP-Rad51 focus formation was nearly normal in brh2 mutant cells expressing a representative Brh2 variant with the C-terminal domain deleted. These findings suggest that the N-terminal region of Brh2 has an innate ability to organize Rad51. Survival after DNA damage was almost fully restored by a chimeric form of Brh2 having a DNA-binding domain from RPA70 fused to the Brh2 N-terminal domain, but Rad51 focus formation and mitotic recombination were elevated above wild-type levels. The results provide evidence for a mechanism in which Dss1 activates a Brh2-Rad51 complex and balances a finely regulated recombinational repair system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.