ArticleProceedings of the National Academy of Sciences of the United States of America2005
FRET measurements of cell-traction forces and nano-scale clustering of adhesion ligands varied by substrate stiffness.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
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Who cites it
101 citing papers in PubMed.
- 3D matrix stiffness drives energy metabolism to orchestrate stem cell osteogenesis via microtubule acetylation and mitochondrial dynamics.Bioactive materials · 2026Article
- Emerging engineering strategies in bone organoids: From biomimetic scaffolds to dynamic microenvironmental stimulation.Bioactive materials · 2026Review
- Microscale Mechanical Cues in Hydrogels: Engineering Strategies to Modulate Cell Fates in Three Dimensions.Cell biomaterials · 2026Article
- Mechanical and Physical Characterization of a Biphasic 3D Printed Silk-Infilled Scaffold for Osteochondral Tissue Engineering.ACS biomaterials science & engineering · 2024Article
- The influence of biophysical niche on tumor-associated macrophages in liver cancer.Hepatology communications · 2024Review
- Multimodal mechano-microscopy reveals mechanical phenotypes of breast cancer spheroids in three dimensions.APL bioengineering · 2024Article
- A homeostatic role of nucleus-actin filament coupling in the regulation of cellular traction forces in fibroblasts.Biomechanics and modeling in mechanobiology · 2024Article
- Towards single cell encapsulation for precision biology and medicine.Advanced drug delivery reviews · 2023Review
- A machine learning approach to predict cellular mechanical stresses in response to chemical perturbation.Biophysical journal · 2023Article
- Cell-extracellular matrix mechanotransduction in 3D.Nature reviews. Molecular cell biology · 2023Review
- Submicron-Sized In Situ Osmotic Pressure Sensors for In Vitro Applications in Biology.Advanced healthcare materials · 2023Article
- May the force be with your (immune) cells: an introduction to traction force microscopy in Immunology.Frontiers in immunology · 2022Review
- Engineering Modular 3D Liver Culture MicroenvironmentsAdvanced nanobiomed research · 2022Article
- Influence of Cellular Microenvironment on Human Articular Chondrocyte Cell Signaling.Cartilage · 2021Article
- Matrix biophysical cues direct mesenchymal stromal cell functions in immunity.Acta biomaterialia · 2021Review
- Review
- Substratum stiffness tunes membrane voltage in mammary epithelial cells.Journal of cell science · 2021Article
- Extracellular matrix stiffness determines DNA repair efficiency and cellular sensitivity to genotoxic agents.Science advances · 2020Article
- Customizable, engineered substrates for rapid screening of cellular cues.Biofabrication · 2020Article
- Extending the Capabilities of Molecular Force Sensors via DNA Nanotechnology.Critical reviews in biomedical engineering · 2020Review
41 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
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Abstract
The mechanical properties of cell adhesion substrates regulate cell phenotype, but the mechanism of this relation is currently unclear. It may involve the magnitude of traction force applied by the cell, and/or the ability of the cells to rearrange the cell adhesion molecules presented from the material. In this study, we describe a FRET technique that can be used to evaluate the mechanics of cell-material interactions at the molecular level and simultaneously quantify the cell-based nanoscale rearrangement of the material itself. We found that these events depended on the mechanical rigidity of the adhesion substrate. Furthermore, both the proliferation and differentiation of preosteoblasts (MC3T3-E1) correlated to the magnitude of force that cells generate to cluster the cell adhesion ligands, but not the extent of ligand clustering. Together, these data demonstrate the utility of FRET in analyzing cell-material interactions, and suggest that regulation of phenotype with substrate stiffness is related to alterations in cellular traction forces.
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