ArticleNucleic acids research2005
Chemical engineering of the peptidyl transferase center reveals an important role of the 2'-hydroxyl group of A2451.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 86 citations in OpenAlex.
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- Assembly of the bacterial ribosome with circularly permuted rRNA.Nucleic acids research · 2024Article
- Assembly of the Bacterial Ribosome with Circularly Permuted rRNA.bioRxiv : the preprint server for biology · 2024Article
- Roles of ribosomal RNA in health and disease.Frontiers in RNA research · 2023Article
- 1-Deazaguanosine-Modified RNA: The Missing Piece for Functional RNA Atomic Mutagenesis.Journal of the American Chemical Society · 2022Article
- The Peptidyl Transferase Center: a Window to the Past.Microbiology and molecular biology reviews : MMBR · 2021Article
- Impact of 3-deazapurine nucleobases on RNA properties.Nucleic acids research · 2021Article
- Oxidative Modifications of RNA and Its Potential Roles in Biosystem.Frontiers in molecular biosciences · 2021Review
- Exploring Allosteric Signaling in the Exit Tunnel of the Bacterial Ribosome by Molecular Dynamics Simulations and Residue Network Model.Frontiers in molecular biosciences · 2020Article
- A tRNA half modulates translation as stress response in Trypanosoma brucei.Nature communications · 2019Article
- Context-Specific Action of Ribosomal Antibiotics.Annual review of microbiology · 2018Review
- Oxidative stress damages rRNA inside the ribosome and differentially affects the catalytic center.Nucleic acids research · 2018Article
- Atomic mutagenesis of stop codon nucleotides reveals the chemical prerequisites for release factor-mediated peptide release.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Critical 23S rRNA interactions for macrolide-dependent ribosome stalling on the ErmCL nascent peptide chain.Nucleic acids research · 2017Article
- Induced fit of the peptidyl-transferase center of the ribosome and conformational freedom of the esterified amino acids.RNA (New York, N.Y.) · 2017Article
- Atomic mutagenesis at the ribosomal decoding site.RNA biology · 2017Article
- Context-specific inhibition of translation by ribosomal antibiotics targeting the peptidyl transferase center.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Proximal disruptor aided ligation (ProDAL) of kilobase-long RNAs.RNA biology · 2016Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The main enzymatic reaction of the large ribosomal subunit is peptide bond formation. Ribosome crystallography showed that A2451 of 23S rRNA makes the closest approach to the attacking amino group of aminoacyl-tRNA. Mutations of A2451 had relatively small effects on transpeptidation and failed to unequivocally identify the crucial functional group(s). Here, we employed an in vitro reconstitution system for chemical engineering the peptidyl transferase center by introducing non-natural nucleosides at position A2451. This allowed us to investigate the peptidyl transfer reaction performed by a ribosome that contained a modified nucleoside at the active site. The main finding is that ribosomes carrying a 2'-deoxyribose at A2451 showed a compromised peptidyl transferase activity. In variance, adenine base modifications and even the removal of the entire nucleobase at A2451 had only little impact on peptide bond formation, as long as the 2'-hydroxyl was present. This implicates a functional or structural role of the 2'-hydroxyl group at A2451 for transpeptidation.
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