ArticleNucleic acids research2005
Competitive enzymatic reaction to control allele-specific extensions.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Terminator-free template-independent enzymatic DNA synthesis for digital information storage.Nature communications · 2019Article
- Recombinant expression library of Pyrococcus furiosus constructed by high-throughput cloning: a useful tool for functional and structural genomics.Frontiers in microbiology · 2015Article
- Narrow genetic basis for the Australian dingo confirmed through analysis of paternal ancestry.Genetica · 2012Article
- Targeted transcript profiling by sequencing.Scientific reports · 2012Article
- Quantitative evaluation of oligonucleotide surface concentrations using polymerization-based amplification.Analytical and bioanalytical chemistry · 2008Article
- Screening of common CYP1B1 mutations in Iranian POAG patients using a microarray-based PrASE protocol.Molecular vision · 2008Article
- Comparison of PrASE and Pyrosequencing for SNP Genotyping.BMC genomics · 2006Article
- Single-tube-genotyping of gastric cancer related SNPs by directly using whole blood and paper-dried blood as starting material.World journal of gastroenterology · 2006Article
- Creating advanced multifunctional biosensors with surface enzymatic transformations.Langmuir : the ACS journal of surfaces and colloids · 2006Article
- Algorithm for automatic genotype calling of single nucleotide polymorphisms using the full course of TaqMan real-time data.Nucleic acids research · 2006Article
- Tri-nucleotide threading for parallel amplification of minute amounts of genomic DNA.Nucleic acids research · 2006Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we present a novel method for SNP genotyping based on protease-mediated allele-specific primer extension (PrASE), where the two allele-specific extension primers only differ in their 3'-positions. As reported previously [Ahmadian,A., Gharizadeh,B., O'Meara,D., Odeberg,J. and Lundeberg,J. (2001), Nucleic Acids Res., 29, e121], the kinetics of perfectly matched primer extension is faster than mismatched primer extension. In this study, we have utilized this difference in kinetics by adding protease, a protein-degrading enzyme, to discriminate between the extension reactions. The competition between the polymerase activity and the enzymatic degradation yields extension of the perfectly matched primer, while the slower extension of mismatched primer is eliminated. To allow multiplex and simultaneous detection of the investigated single nucleotide polymorphisms (SNPs), each extension primer was given a unique signature tag sequence on its 5' end, complementary to a tag on a generic array. A multiplex nested PCR with 13 SNPs was performed in a total of 36 individuals and their alleles were scored. To demonstrate the improvements in scoring SNPs by PrASE, we also genotyped the individuals without inclusion of protease in the extension. We conclude that the developed assay is highly allele-specific, with excellent multiplex SNP capabilities.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.