ArticleCell calcium1992
Fluorescence lifetime imaging of calcium using Quin-2.
Article in Cell calcium, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 122 citations in OpenAlex.
- Two-photon autofluorescence lifetime assay of rabbit photoreceptors and retinal pigment epithelium during light-dark visual cycles in rabbit retina.Biomedical optics express · 2024Article
- Development status of novel spectral imaging techniques and application to traditional Chinese medicine.Journal of pharmaceutical analysis · 2023Review
- Remote Measurements of Tear Electrolyte Concentrations on Both Sides of an Inserted Contact Lens.Chemosensors (Basel, Switzerland) · 2023Article
- Simple and Robust Deep Learning Approach for Fast Fluorescence Lifetime Imaging.Sensors (Basel, Switzerland) · 2022Article
- NAD(P)H fluorescence lifetime imaging of live intestinal nematodes reveals metabolic crosstalk between parasite and host.Scientific reports · 2022Article
- Single-Photon, Time-Gated, Phasor-Based Fluorescence Lifetime Imaging through Highly Scattering Medium.ACS photonics · 2020Article
- New luminescence lifetime macro-imager based on a Tpx3Cam optical camera.Biomedical optics express · 2020Article
- Quantitative determination of cellular [NaThe Journal of general physiology · 2019Article
- Contact lens to measure individual ion concentrations in tears and applications to dry eye disease.Analytical biochemistry · 2018Article
- Accurate Rapid Lifetime Determination on Time-Gated FLIM Microscopy with Optical Sectioning.Journal of healthcare engineering · 2018Article
- Effect of Ca²⁺ on the steady-state and time-resolved emission properties of the genetically encoded fluorescent sensor CatchER.The journal of physical chemistry. B · 2015Article
- Asante Calcium Green and Asante Calcium Red--novel calcium indicators for two-photon fluorescence lifetime imaging.PloS one · 2014Article
- Signaling in dendritic spines and spine microdomains.Current opinion in neurobiology · 2012Review
- Nanoparticle PEBBLE sensors for quantitative nanomolar imaging of intracellular free calcium ions.Analytical chemistry · 2012Article
- Time-domain fluorescence lifetime imaging techniques suitable for solid-state imaging sensor arrays.Sensors (Basel, Switzerland) · 2012Article
- Phasor imaging with a widefield photon-counting detector.Journal of biomedical optics · 2012Article
- Increased plasma colloid osmotic pressure facilitates the uptake of therapeutic macromolecules in a xenograft tumor model.Neoplasia (New York, N.Y.) · 2009Article
- Ultrafast Method for the Analysis of Fluorescence Lifetime Imaging Microscopy Data Based on the Laguerre Expansion Technique.IEEE journal of quantum electronics · 2005Article
- Effects of temperature on calcium-sensitive fluorescent probes.Biophysical journal · 2000Article
- Low-frequency modulation sensors using nanosecond fluorophores.Analytical chemistry · 1998Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
We describe the use of a new imaging technology, fluorescence lifetime imaging (FLIM), for the imaging of the calcium concentrations based on the fluorescence lifetime of a calcium indicator. The fluorescence lifetime of Quin-2 is shown to be highly sensitive to [Ca2+]. We create two-dimensional lifetime images using the phase shift and modulation of the Quin-2 in response to intensity-modulated light. The two-dimensional phase and modulation values are obtained using a gain-modulated image intensifier and a slow-scan CCD camera. The lifetime values in the 2D image were verified using standard frequency-domain measurements. Importantly, the FLIM method does not require the probe to display shifts in the excitation or emission spectra, which may allow Ca2+ imaging using other Ca2+ probes not in current widespread use due to the lack of spectral shifts. Fluorescence lifetime imaging can be superior to stationary (steady-state) imaging because lifetimes are independent of the local probe concentration and/or intensity, and should thus be widely applicable to chemical imaging using fluorescence microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.