ArticleBone marrow transplantation2005
Aldehyde dehydrogenase activity as a marker for the quality of hematopoietic stem cell transplants.
Article in Bone marrow transplantation, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 49 citations in OpenAlex.
- Expansion and preservation of the functional activity of adult hematopoietic stem cells cultured ex vivo with a histone deacetylase inhibitor.Stem cells translational medicine · 2020Article
- Aldehyde Dehydrogenases: Not Just Markers, but Functional Regulators of Stem Cells.Stem cells international · 2019Review
- Enhancing human cord blood hematopoietic stem cell engraftment by targeting nuclear hormone receptors.Current opinion in hematology · 2018Review
- Foreskin-derived mesenchymal stromal cells with aldehyde dehydrogenase activity: isolation and gene profiling.BMC cell biology · 2018Article
- Flow Cytometric Aldehyde Dehydrogenase Assay Enables a Fast and Accurate Human Umbilical Cord Blood Hematopoietic Stem Cell Assessment.Turkish journal of haematology : official journal of Turkish Society of Haematology · 2017Article
- Reduced hematopoietic stem cell frequency predicts outcome in acute myeloid leukemia.Haematologica · 2017Article
- Development and validation of a rapid, aldehyde dehydrogenase bright-based cord blood potency assay.Blood · 2016Article
- Infectious dengue vesicles derived from CD61+ cells in acute patient plasma exhibited a diaphanous appearance.Scientific reports · 2015Article
- Epigenetic reprogramming induces the expansion of cord blood stem cells.The Journal of clinical investigation · 2014Article
- Infused neutrophil dose and haematopoietic recovery in patients undergoing autologous transplantation.Bone marrow transplantation · 2014Article
- Timing of peripheral blood stem cell yield: comparison of alternative methods with the classic method for CD34+ cell determination.BioMed research international · 2014Article
- Aldehyde dehydrogenases: from eye crystallins to metabolic disease and cancer stem cells.Chemico-biological interactions · 2013Review
- Is it time to revisit our current hematopoietic progenitor cell quantification methods in the clinic?Bone marrow transplantation · 2012Review
- The enzymatic activity of human aldehyde dehydrogenases 1A2 and 2 (ALDH1A2 and ALDH2) is detected by Aldefluor, inhibited by diethylaminobenzaldehyde and has significant effects on cell proliferation and drug resistance.Chemico-biological interactions · 2012Article
- Surface antigenic profiling of stem cells from human omentum fat in comparison with subcutaneous fat and bone marrow.Cytotechnology · 2012Article
- Article
- Aldehyde dehydrogenase activity identifies a population of human skeletal muscle cells with high myogenic capacities.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- Transportation and cryopreservation may impair haematopoietic stem cell function and engraftment of allogeneic PBSCs, but not BM.Bone marrow transplantation · 2008Article
- Article
- Factors affecting volume reduction and red blood cell depletion of bone marrow on the COBE Spectra cell separator before haematopoietic stem cell transplantation.Bone marrow transplantation · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Taking advantage of fluorescent substrates for their metabolic marker aldehyde dehydrogenase (ALDH), hematopoietic stem cells (HSC) were defined as SSC(lo)ALDH(br) - reflecting their low orthogonal light scattering and bright fluorescence intensity in flow cytometry. Based thereon, we investigated the usefulness of ALDH activity for characterizing HSC graft quality, particularly under stress conditions. We first compared the expression of ALDH vs CD34 in bone marrow and peripheral blood stem cell (PBSC) samples over 7 days. We noted that (i) only ALDH activity but not CD34 expression strongly reflected colony-forming ability over time, and that (ii) PBSC grafts stored at room temperature lost most of their progenitor cells within just 48 h. We then retrospectively related ALDH and CD34 expression as well as granulocyte-macrophage colony-forming units (CFU-GM) potential for 19 cryopreserved allogeneic PBSC grafts to engraftment data. Strikingly, in all six patients who received markedly decreased numbers of SSC(lo)ALDH(br) cells, this was associated not only with almost complete loss of CFU-GM potential but also with delayed establishment/permanent absence of full hematopoietic donor cell chimerism, whereas all other patients showed early complete donor chimerism. In conclusion, we suggest to measure ALDH activity as a surrogate marker for HSC activity, and to transport and store PBSC under controlled cooling conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.