ArticleProceedings of the National Academy of Sciences of the United States of America2005
Spliceosomal introns in the deep-branching eukaryote Trichomonas vaginalis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 92 citations in OpenAlex.
- Long-read transcriptomics corrects Trichomonas vaginalis intron annotations and refines transcript-end features.Parasites & vectors · 2026Article
- Chromosome-level genome assembly of Trichomonas vaginalis strain IR-78 (ATCC 50138).Scientific data · 2025Article
- Redefining the spliceosomal introns of the sexually transmitted parasite Trichomonas vaginalis and its close relative in columbid birds.PLoS pathogens · 2025Article
- Decryption of the survival "black box": gene family expansion promotes the encystment in ciliated protists.BMC genomics · 2024Article
- An Unusual U2AF2 Inhibits Splicing and Attenuates the Virulence of the Human Protozoan ParasiteFrontiers in cellular and infection microbiology · 2022Article
- Patterns of conservation of spliceosomal intron structures and spliceosome divergence in representatives of the diplomonad and parabasalid lineages.BMC evolutionary biology · 2019Article
- The cellular landscape of mid-size noncoding RNA.Wiley interdisciplinary reviews. RNA · 2019Review
- Spliceosomal introns in Trichomonas vaginalis revisited.Parasites & vectors · 2018Article
- Postsplicing-Derived Full-Length Intron Circles in the Protozoan ParasiteFrontiers in cellular and infection microbiology · 2018Article
- The Macronuclear Genome of Stentor coeruleus Reveals Tiny Introns in a Giant Cell.Current biology : CB · 2017Article
- Transcriptomic analysis of diplomonad parasites reveals a trans-spliced intron in a helicase gene inPeerJ · 2017Article
- Trichomonas vaginalis homolog of macrophage migration inhibitory factor induces prostate cell growth, invasiveness, and inflammatory responses.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Origin of spliceosomal introns and alternative splicing.Cold Spring Harbor perspectives in biology · 2014Article
- Reverse transcriptase and intron number evolution.Stem cell investigation · 2014Article
- A phylogenetic study ofOpen journal of animal sciences · 2013Article
- GenePainter: a fast tool for aligning gene structures of eukaryotic protein families, visualizing the alignments and mapping gene structures onto protein structures.BMC bioinformatics · 2013Article
- Box H/ACA snoRNAs are preferred substrates for the trimethylguanosine synthase in the divergent unicellular eukaryote Trichomonas vaginalis.RNA (New York, N.Y.) · 2012Article
- Functional analysis of sequence motifs involved in the polyadenylation of Trichomonas vaginalis mRNAs.Eukaryotic cell · 2012Article
- Origin and evolution of spliceosomal introns.Biology direct · 2012Review
- Widespread recurrent evolution of genomic features.Genome biology and evolution · 2012Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
Eukaryotes have evolved elaborate splicing mechanisms to remove introns that would otherwise destroy the protein-coding capacity of genes. Nuclear premRNA splicing requires sequence motifs in the intron and is mediated by a ribonucleoprotein complex, the spliceosome. Here we demonstrate the presence of a splicing apparatus in the protist Trichomonas vaginalis and show that RNA motifs found in yeast and metazoan introns are required for splicing. We also describe the first introns in this deep-branching lineage. The positions of these introns are often conserved in orthologous genes, indicating they were present in a common ancestor of trichomonads, yeast, and metazoa. All examined T. vaginalis introns have a highly conserved 12-nt 3' splice-site motif that encompasses the branch point and is necessary for splicing. This motif is also found in the only described intron in a gene from another deep-branching eukaryote, Giardia intestinalis. These studies demonstrate the conservation of intron splicing signals across large evolutionary distances, reveal unexpected motif conservation in deep-branching lineages that suggest a simplified mechanism of splicing in primitive unicellular eukaryotes, and support the presence of introns in the earliest eukaryote.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.