ArticleBiomagnetic research and technology2005
Activity of glucose oxidase functionalized onto magnetic nanoparticles.
Article in Biomagnetic research and technology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 121 citations in OpenAlex.
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- Enzymatic Polymer Brush Interfaces for Electrochemical Sensing in Biofluids.ACS applied bio materials · 2025Article
- Critical Parameters in an Enzymatic Way to Obtain the Unsweet Lactose-Free Milk Using Catalase and Glucose Oxidase Co-Encapsulated into Hydrogel with Chemical Cross-Linking.Foods (Basel, Switzerland) · 2022Article
- Synthesis and Comparative Studies of Glucose Oxidase Immobilized on FeNanomaterials (Basel, Switzerland) · 2022Article
- Industrial applications of immobilized nano-biocatalysts.Bioprocess and biosystems engineering · 2022Review
- Metabolic Syndrome-An Emerging Constellation of Risk Factors: Electrochemical Detection Strategies.Sensors (Basel, Switzerland) · 2019Review
- A biosensor capable of identifying low quantities of breast cancer cells by electrical impedance spectroscopy.Scientific reports · 2019Article
- High-power biofuel cells based on three-dimensional reduced graphene oxide/carbon nanotube micro-arrays.Microsystems & nanoengineering · 2019Article
- Gold nanoparticles - an optical biosensor for RNA quantification for cancer and neurologic disorders diagnosis.International journal of nanomedicine · 2018Article
- Characterization of ferrite nanoparticles for preparation of biocomposites.Beilstein journal of nanotechnology · 2017Article
- Application of iron magnetic nanoparticles in protein immobilization.Molecules (Basel, Switzerland) · 2014Review
- Enzyme immobilization: an update.Journal of chemical biology · 2013Review
- Aluminum gallium nitride (GaN)/GaN high electron mobility transistor-based sensors for glucose detection in exhaled breath condensate.Journal of diabetes science and technology · 2010Article
- A nanoparticle-based immobilization assay for prion-kinetics study.Journal of nanobiotechnology · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMagnetic nanoparticles have been significantly used for coupling with biomolecules, due to their unique properties.
methodsMagnetic nanoparticles were synthesized by thermal co-precipitation of ferric and ferrous chloride using two different base solutions. Glucose oxidase was bound to the particles by direct attachment via carbodiimide activation or by thiophene acetylation of magnetic nanoparticles. Transmission electron microscopy was used to characterize the size and structure of the particles while the binding of glucose oxidase to the particles was confirmed using Fourier transform infrared spectroscopy.
resultsThe direct binding of glucose oxidase via carbodiimide activity was found to be more effective, resulting in bound enzyme efficiencies between 94-100% while thiophene acetylation was 66-72% efficient. Kinetic and stability studies showed that the enzyme activity was more preserved upon binding onto the nanoparticles when subjected to thermal and various pH conditions. The overall activity of glucose oxidase was improved when bound to magnetic nanoparticles
conclusionBinding of enzyme onto magnetic nanoparticles via carbodiimide activation is a very efficient method for developing bioconjugates for biological applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.