ArticlePlant cell reports2005
A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques.
Article in Plant cell reports, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
73 citing papers in PubMed, 251 citations in OpenAlex.
- Molecular regulators of regeneration and strategies for overcoming genotype-dependent recalcitrance in wheat (Triticum aestivum).Communications biology · 2026Review
- Endogenous expression of the wound signal peptide ZmREF1 enhances Agrobacterium-mediated transformation of maize inbred B104.Plant cell reports · 2026Article
- Efficient Agrobacterium-Mediated Transformation of Green Arabidopsis Suspension Cells.Biotechnology journal · 2025Article
- Host-Induced Gene Silencing of theToxins · 2024Article
- Tobacco Plant: A Novel and Promising Heterologous Bioreactor for the Production of Recombinant Bovine Chymosin.Molecular biotechnology · 2024Article
- Optimizing genome editing efficiency in wheat: Effects of heat treatments and different promoters for single guide RNA expression.Plant biotechnology (Tokyo, Japan) · 2023Article
- Effect of T-DNA Integration on Growth of TransgenicInternational journal of molecular sciences · 2023Article
- Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing.International journal of molecular sciences · 2023Review
- An EfficientPlants (Basel, Switzerland) · 2023Article
- A Genotype-Independent, Simple, Effective and Efficient in PlantaMethods and protocols · 2022Article
- In vitro regeneration and Agrobacterium-mediated genetic transformation of Dragon's Head plant (Lallemantia iberica).Scientific reports · 2022Article
- Gene Editing Technologies for Sugarcane Improvement: Opportunities and Limitations.Sugar tech : an international journal of sugar crops & related industries · 2022Article
- Article
- Resistance to Aflatoxin Accumulation in Maize Mediated by Host-Induced Silencing of theJournal of fungi (Basel, Switzerland) · 2021Article
- Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants.International journal of molecular sciences · 2021Review
- A competence of embryo-derived tissues of tetraploid cultivated wheat species Triticum dicoccum and Triticum timopheevii for efficient and stable transgenesis mediated by particle inflow gun.BMC plant biology · 2020Article
- Plastid Transformation: How Does it Work? Can it Be Applied to Crops? What Can it Offer?International journal of molecular sciences · 2020Review
- The present and potential future methods for delivering CRISPR/Cas9 components in plants.Journal, genetic engineering & biotechnology · 2020Review
- Low-temperature pretreatment of explants and high maltose concentration during callus culture improves particle-bombardment-mediated stable transgene expression in common wheat.Plant biotechnology (Tokyo, Japan) · 2020Article
- In Vitro Tissue Culture inInternational journal of molecular sciences · 2020Review
13 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two barley transformation systems, Agrobacterium-mediated and particle bombardment, were compared in terms of transformation efficiency, transgene copy number, expression, inheritance and physical structure of the transgenic loci using fluorescence in situ hybridisation (FISH). The efficiency of Agrobacterium-mediated transformation was double that obtained with particle bombardment. While 100% of the Agrobacterium-derived lines integrated between one and three copies of the transgene, 60% of the transgenic lines derived by particle bombardment integrated more than eight copies of the transgene. In most of the Agrobacterium-derived lines, the integrated T-DNA was stable and inherited as a simple Mendelian trait. Transgene silencing was frequently observed in the T1 populations of the bombardment-derived lines. The FISH technique was able to reveal additional details of the transgene integration site. For the efficient production of transgenic barley plants, with stable transgene expression and reduced silencing, the Agrobacterium-mediated method appears to offer significant advantages over particle bombardment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.