ArticleProceedings of the National Academy of Sciences of the United States of America2005
The interaction between sigma70 and the beta-flap of Escherichia coli RNA polymerase inhibits extension of nascent RNA during early elongation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Recent advances in mycobacterial transcription: insights beyond the general pathway.Journal of bacteriology · 2025Review
- In transcription antitermination by Qλ, NusA induces refolding of Qλ to form a nozzle that extends the RNA polymerase RNA-exit channel.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Structural and mechanistic basis of σ-dependent transcriptional pausing.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Transcriptional control of mycobacterial DNA damage response by sigma adaptation.Science advances · 2021Article
- Universal functions of the σ finger in alternative σ factors during transcription initiation by bacterial RNA polymerase.RNA biology · 2021Article
- Transcription complexes as RNA chaperones.Transcription · 2021Article
- H-NS-like proteins in Pseudomonas aeruginosa coordinately silence intragenic transcription.Molecular microbiology · 2021Article
- Extracytoplasmic Function σ Factors as Tools for Coordinating Stress Responses.International journal of molecular sciences · 2021Review
- Context-Dependent Action of Scc4 Reinforces Control of the Type III Secretion System.Journal of bacteriology · 2020Article
- The Role of Pyrophosphorolysis in the Initiation-to-Elongation Transition by E. coli RNA Polymerase.Journal of molecular biology · 2019Article
- Genetic Diversity and Functional Analysis of Sigma Factors inEvolutionary bioinformatics online · 2018Article
- σ38-dependent promoter-proximal pausing by bacterial RNA polymerase.Nucleic acids research · 2017Article
- Visualizing the phage T4 activated transcription complex of DNA and E. coli RNA polymerase.Nucleic acids research · 2016Article
- Nascent RNA length dictates opposing effects of NusA on antitermination.Nucleic acids research · 2016Article
- Article
- The evolutionarily conserved Pol II flap loop contributes to proper transcription termination on short yeast genes.Cell reports · 2014Article
- Competence for genetic transformation in Streptococcus pneumoniae: mutations in σA bypass the comW requirement.Journal of bacteriology · 2014Article
- Regulation of promoter-proximal transcription elongation: enhanced DNA scrunching drives λQ antiterminator-dependent escape from a σ70-dependent pause.Nucleic acids research · 2014Article
- Distinct functions of the RNA polymerase σ subunit region 3.2 in RNA priming and promoter escape.Nucleic acids research · 2014Article
- Distinct functions of regions 1.1 and 1.2 of RNA polymerase σ subunits from Escherichia coli and Thermus aquaticus in transcription initiation.The Journal of biological chemistry · 2012Article
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7 authors.
Funding
Abstract
The sigma-subunit of bacterial RNA polymerase (RNAP) is required for promoter-specific transcription initiation. This function depends on specific intersubunit interactions that occur when sigma associates with the RNAP core enzyme to form RNAP holoenzyme. Among these interactions, that between conserved region 4 of sigma and the flap domain of the RNAP beta-subunit (beta-flap) is critical for recognition of the major class of bacterial promoters. Here, we describe the isolation of amino acid substitutions in region 4 of Escherichia coli sigma(70) that have specific effects on the sigma(70) region 4/beta-flap interaction, either weakening or strengthening it. Using these sigma(70) mutants, we demonstrate that the sigma region 4/beta-flap interaction also can affect events occurring downstream of transcription initiation during early elongation. Specifically, our results provide support for a structure-based proposal that, when bound to the beta-flap, sigma region 4 presents a barrier to the extension of the nascent RNA as it emerges from the RNA exit channel. Our findings support the view that the transition from initiation to elongation involves a staged disruption of sigma-core interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.