ArticleInternational journal of legal medicine2005
Real-time quantitative PCR assay for the detection of Helicobacter pylori: no association with sudden infant death syndrome.
Article in International journal of legal medicine, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Post-mortem diagnosis of malaria in decomposed and embalmed body.Forensic science, medicine, and pathology · 2025Article
- The Inhibitory Effect of Mouse Gastric DNA on Amplification of Helicobacter pylori Genomic DNA in Quantitative PCRIranian biomedical journal · 2019Article
- Development of defined microbial population standards using fluorescence activated cell sorting for the absolute quantification of S. aureus using real-time PCR.Molecular biotechnology · 2012Article
- Ultrasound-accelerated formalin fixation improves the preservation of nucleic acids extraction in histological sections.International journal of legal medicine · 2009Article
- QT interval prolongation and risk for cardiac events in genotyped LQTS-index children.European journal of pediatrics · 2009Article
- Correlation of inter-individual variations of amitriptyline metabolism examined in hairs with CYP2C19 and CYP2D6 polymorphisms.International journal of legal medicine · 2008Article
- Successful RNA extraction from various human postmortem tissues.International journal of legal medicine · 2007Article
- Sudden infant death syndrome and long QT syndrome: an epidemiological and genetic study.International journal of legal medicine · 2006Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Helicobacter pylori infection is known to be one of the most common chronic infectious diseases in humans. Recently, a hypothesis was proposed that H. pylori infection could be a frequent cause for sudden infant death syndrome (SIDS). We have investigated this postulated association by examining formalin-fixed paraffin-embedded gastric tissues of a retrospective cohort of 94 SIDS cases: The presence of H. pylori was inferred from a newly developed real-time quantitative PCR assay with SYBR Green I detection. This assay is based on the amplification of the single-copy H. pylori-specific glmM gene. Accuracy and precision were verified using a plasmid containing a 977-bp fragment of this glmM gene. The assay was very sensitive, and as few as 30 template copies per PCR reaction could be detected even in the presence of excess human DNA. The assay was validated on mucosal biopsy samples of patients with known H. pylori infections. Interfering effects due to SIDS gastric tissue were excluded. Only two (2.1%) of the SIDS samples yielded H. pylori DNA copy numbers and only beyond the lowest standard concentration. These results could be confirmed independently by immunohistochemistry using an H. pylori-specific antibody. Thus, an infection by H. pylori is very rare in cases of SIDS, and thus the postulated association of H. pylori infection and SIDS cannot be confirmed.
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