Evidence map›Paper›PMID 15757550›Full record

ArticleEmerging infectious diseases2005

Rapid identification of emerging pathogens: coronavirus.

Rangarajan Sampath, Steven A Hofstadler, Lawrence B Blyn, Mark W Eshoo, Thomas A Hall, Christian Massire, Harold M Levene, James C Hannis, Patina M Harrell, Benjamin Neuman and 9 more

Open access · diamondAbstract read
In one paragraph

Article in Emerging infectious diseases, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
60citing papers in PubMed, 1 pooled it
4.2field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

60 citing papers in PubMed, 1 synthesis or guideline pooled it, 101 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Article
  4. Review
  5. Review
  6. Laboratory diagnosis of COVID-19 in China: A review of challenging cases and analysis.Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi · 2021
    Review
  7. MASS SPECTROMETRY IN VIROLOGICAL SCIENCES.Mass spectrometry reviews · 2020
    Review
  8. Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Sterile and microbial-associated intra-amniotic inflammation in preterm prelabor rupture of membranes.The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians · 2015
    Article
  14. Review
  15. Sterile intra-amniotic inflammation in asymptomatic patients with a sonographic short cervix: prevalence and clinical significance.The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians · 2015
    Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Differentiating microbial forensic qPCR target and control products by electrospray ionization mass spectrometry.Biosecurity and bioterrorism : biodefense strategy, practice, and science · 2013
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 2 institutions in 1 country.

Rangarajan SampathIbis Therapeutics, 1891 Rutherford Ct, Carlsbad, CA 92008, USA. rsampath@isisph.com
Steven A Hofstadler
Lawrence B Blyn
Mark W Eshoo
Thomas A Hall
Christian Massire
Harold M Levene
James C Hannis
Patina M Harrell
Benjamin Neuman
Michael J Buchmeier
Yun Jiang
Raymond Ranken
Jared J Drader
Vivek Samant
Richard H Griffey
John A McNeil
Stanley T Crooke
David J Ecker
Ionis Pharmaceuticals (United States) · USScripps Research Institute · US

Funding

Virology of the Central Nervous SystemT32NS041219 · NINDS · SCRIPPS RESEARCH INSTITUTE, THE · PI ELDER, JOHN H · 2001 to 2010
$2.4M
MECHANISMS OF MURINE CORONAVIRUS NEUROVIRULENCER01AI025913 · NIAID · SCRIPPS RESEARCH INSTITUTE · PI BUCHMEIER, MICHAEL · 1988 to 2003
$1.3M
NIAID NIH HHS AI 25913NIAID NIH HHS R01 AI025913NINDS NIH HHS T32 NS041219NINDS NIH HHS T32 NS 41219
6 · The paper itself

Abstract

We describe a new approach for infectious disease surveillance that facilitates rapid identification of known and emerging pathogens. The process uses broad-range polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry for accurate mass measurements of PCR products, and base composition signature analysis to identify organisms in a sample. We demonstrate this principle by using 14 isolates of 9 diverse Coronavirus spp., including the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). We show that this method could identify and distinguish between SARS and other known CoV, including the human CoV 229E and OC43, individually and in a mixture of all 3 human viruses. The sensitivity of detection, measured by using titered SARS-CoV spiked into human serum, was approximate, equals1 PFU/mL. This approach, applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens, is capable of automated analysis of >900 PCR reactions per day.

Indexed as

AnimalsBase SequenceCommunicable Diseases, EmergingCoronaviridaeCoronaviridae InfectionsHumansPolymerase Chain ReactionPopulation SurveillanceRNA, ViralSensitivity and SpecificitySpectrometry, Mass, Electrospray IonizationRNA, Viral

Identifiers

PMID15757550
PMCPMC3298233
OpenAlexW2012080636

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.