ArticlePLoS biology2005
Functional evolution of a cis-regulatory module.
Article in PLoS biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 124 papers, 1 of them a synthesis that pooled it.
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Who cites it
124 citing papers in PubMed, 1 synthesis or guideline pooled it.
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- The combinatorial binding syntax of transcription factors in forebrain-specific enhancers.Biology open · 2025Article
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- The Density of Regulatory Information Is a Major Determinant of Evolutionary Constraint on Noncoding DNA in Drosophila.Molecular biology and evolution · 2024Article
- Increased chromatin accessibility promotes the evolution of a transcriptional silencer inScience advances · 2023Article
- regCNN: identifyingComputational and structural biotechnology journal · 2022Article
- Repression precedes independent evolutionary gains of a highly specific gene expression pattern.Cell reports · 2021Article
- Evidence from simulation studies for selective constraints on the codon usage of the Angiosperm psbA gene.PLoS computational biology · 2021Article
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- Transcriptional Enhancers inGenetics · 2020Review
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- Lineage specific conservation of cis-regulatory elements in Cytokinin Response Factors.Scientific reports · 2019Article
- Studying Transcriptional Enhancers: The Founder Fallacy, Validation Creep, and Other Biases.Trends in genetics : TIG · 2019Review
- Functional Conservation of a Developmental Switch in Mammals since the Jurassic Age.Molecular biology and evolution · 2019Article
- Hunchback is counter-repressed to regulate even-skipped stripe 2 expression in Drosophila embryos.PLoS genetics · 2018Article
- ChIP-ping the branches of the tree: functional genomics and the evolution of eukaryotic gene regulation.Briefings in functional genomics · 2018Review
- Differential gene expression during early development in recently evolved and sympatric Arctic charr morphs.PeerJ · 2018Article
64 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
Abstract
Lack of knowledge about how regulatory regions evolve in relation to their structure-function may limit the utility of comparative sequence analysis in deciphering cis-regulatory sequences. To address this we applied reverse genetics to carry out a functional genetic complementation analysis of a eukaryotic cis-regulatory module-the even-skipped stripe 2 enhancer-from four Drosophila species. The evolution of this enhancer is non-clock-like, with important functional differences between closely related species and functional convergence between distantly related species. Functional divergence is attributable to differences in activation levels rather than spatiotemporal control of gene expression. Our findings have implications for understanding enhancer structure-function, mechanisms of speciation and computational identification of regulatory modules.
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