ArticleImmunogenetics2005
Characterization and expression analysis of CD3varepsilon and CD3gamma/delta in fugu, Takifugu rubripes.
Article in Immunogenetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Cluster of differentiation antigens: essential roles in the identification of teleost fish T lymphocytes.Marine life science & technology · 2022Review
- Molecular and cellular characterization of European sea bass CD3εCell and tissue research · 2021Article
- Review
- Peculiar Expression of CD3-Epsilon in Kidney of Ginbuna Crucian Carp.Frontiers in immunology · 2018Article
- T Cells in Fish.Biology · 2015Review
- The Mucosal Immune System of Teleost Fish.Biology · 2015Review
- Genome complexity in the coelacanth is reflected in its adaptive immune system.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2014Article
- Molecular and biochemical characterization of the Mexican axolotl CD3 (CD3ε and CD3γ/δ).Immunogenetics · 2011Article
- CD3γ/δ in sea bass (Dicentrarchus labrax): Molecular characterization and expression analysis.Results in immunology · 2011Article
- Evidence of positive selection on the Atlantic salmon CD3gammadelta gene.Immunogenetics · 2007Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CD3 is an essential component of the CD3-TCR complex. In this report, we describe the cloning, characterization, and expression analysis of the CD3varepsilon and CD3gamma/delta chain genes from fugu, Takifugu rubripes. Two distinct CD3varepsilon homologue cDNAs, designated as CD3varepsilon-1 and CD3varepsilon-2, and a CD3gamma/delta homologue cDNA were isolated from the fugu thymus. The deduced amino acid sequences of these cDNAs exhibit conserved essential CD3 chain motifs and overall structures. RT-PCR analysis demonstrated that the CD3varepsilon and CD3gamma/delta genes were expressed in lymphoid organs (e.g. thymus, head kidney, trunk kidney and spleen), mucosal tissues (gill, skin, and intestine), and peripheral blood leucocytes (PBL). The CD3 and TCRalpha genes were expressed only in the surface IgM- population, which were separated from PBL using an anti-fugu IgM monoclonal antibody. In addition, in situ hybridization confirmed that CD3-expressing cells were distributed randomly in the head kidney, trunk kidney, and spleen, but in the thymus were restricted to the lymphoid outer zone and epithelioid inner zone only. Collectively, these results suggest that CD3 molecules are useful markers for the identification of T cells in teleost fish. The present study thus provides a critical step in identifying T cells in this model organism.
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