ArticleWorld journal of gastroenterology2005
Spatial organization of bacterial flora in normal and inflamed intestine: a fluorescence in situ hybridization study in mice.
Article in World journal of gastroenterology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 141 papers, 4 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
141 citing papers in PubMed, 4 syntheses or guidelines pooled it, 285 citations in OpenAlex.
- The effects of dairy and dairy derivatives on the gut microbiota: a systematic literature review.Gut microbes · 2020Pooled it
- Relationship between pouch microbiota and pouchitis following restorative proctocolectomy for ulcerative colitis.World journal of gastroenterology · 2014Pooled it
- Guideline
- Association Between Sex Hormone Levels and Gut Microbiota Composition and Diversity-A Systematic Review.Journal of clinical gastroenterologyPooled it
- Necrotic enteritis due to simultaneous infection with Isospora suis and clostridia in newborn piglets and its prevention by early treatment with toltrazuril.Parasitology research · 2012Trial
- Review
- Fusobacterium nucleatum, succinate signaling, and immunotherapy resistance in colorectal cancer: clinical relevance and translational opportunities.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2026Review
- A Developmental Perspective on the Intestinal Microbiota in Crohn's Disease.International journal of molecular sciences · 2026Review
- The role of gut microbiome in the pathophysiology of PTSD, depression, and anxiety disorders.Gut microbes reports · 2026Review
- Microbiota decolonization of bacterial pathogens in pediatric surgery-related intestinal disorders: Insights on current strategies and future outlook.World journal of clinical pediatrics · 2025Review
- Comparative analysis of composition and spatial variations in the foregut microbiota of male and female donkeys.Frontiers in microbiology · 2025Article
- Air-Pollution-Mediated Microbial Dysbiosis in Health and Disease: Lung-Gut Axis and Beyond.Journal of xenobiotics · 2024Review
- A versatile tissue-rolling technique for spatial-omics analyses of the entire murine gastrointestinal tract.Nature protocols · 2024Review
- Spatial host-microbiome sequencing reveals niches in the mouse gut.Nature biotechnology · 2024Article
- Mathematical Modeling of Vedolizumab Treatment's Effect on Microbiota and Intestinal Permeability in Inflammatory Bowel Disease Patients.Bioengineering (Basel, Switzerland) · 2024Article
- Unraveling the Microbiome-Human Body Axis: A Comprehensive Examination of Therapeutic Strategies, Interactions and Implications.International journal of molecular sciences · 2024Review
- Rab7-dependent regulation of goblet cell protein CLCA1 modulates gastrointestinal homeostasis.eLife · 2024Article
- The Modulatory Effects of Curcumin on the Gut Microbiota: A Potential Strategy for Disease Treatment and Health Promotion.Microorganisms · 2024Review
- Significance of Gut Microbiota on Graves' Disease.International journal of general medicine · 2024Review
- What we need to know about the germ-free animal models.AIMS microbiology · 2024Review
81 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo study the role of intestinal flora in inflammatory bowel disease (IBD).
methodsThe spatial organization of intestinal flora was investigated in normal mice and in two models of murine colitis using fluorescence in situ hybridization.
resultsThe murine small intestine was nearly bacteria-free. The normal colonic flora was organized in three distinct compartments (crypt, interlaced, and fecal), each with different bacterial compositions. Crypt bacteria were present in the cecum and proximal colon. The fecal compartment was composed of homogeneously mixed bacterial groups that directly contacted the colonic wall in the cecum but were separated from the proximal colonic wall by a dense interlaced layer. Beginning in the middle colon, a mucus gap of growing thickness physically separated all intestinal bacteria from contact with the epithelium. Colonic inflammation was accompanied with a depletion of bacteria within the fecal compartment, a reduced surface area in which feces had direct contact with the colonic wall, increased thickness and spread of the mucus gap, and massive increases of bacterial concentrations in the crypt and interlaced compartments. Adhesive and infiltrative bacteria were observed in inflamed colon only, with dominant Bacteroides species.
conclusionThe proximal and distal colons are functionally different organs with respect to the intestinal flora, representing a bioreactor and a segregation device. The highly organized structure of the colonic flora, its specific arrangement in different colonic segments, and its specialized response to inflammatory stimuli indicate that the intestinal flora is an innate part of host immunity that is under complex control.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.