ArticleArthritis and rheumatism2005
Hyaluronan oligosaccharide-induced activation of transcription factors in bovine articular chondrocytes.
Article in Arthritis and rheumatism, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- The mechanism of action for hyaluronic acid treatment in the osteoarthritic knee: a systematic review.BMC musculoskeletal disorders · 2015Pooled it
- Hyaluronic Acid: Known for Almost a Century, but Still in Vogue.Pharmaceutics · 2022Review
- Usefulness of serum hyaluronic acid levels as a predictor of incidence of hand osteoarthritis analyzed by longitudinal analysis from the Iwaki cohort.Scientific reports · 2021Article
- A Hyaluronan-binding Peptide (P15-1) Reduces Inflammatory and Catabolic Events in IL-1β-treated Human Articular Chondrocytes.Scientific reports · 2020Article
- Role of Hyaluronan in Inflammatory Effects on Human Articular Chondrocytes.Inflammation · 2019Article
- The pericellular hyaluronan of articular chondrocytes.Matrix biology : journal of the International Society for Matrix Biology · 2019Review
- 4-Methylumbelliferone Diminishes Catabolically Activated Articular Chondrocytes and Cartilage Explants via a Mechanism Independent of Hyaluronan Inhibition.The Journal of biological chemistry · 2016Article
- Hyaluronan Inhibits Tlr-4-Dependent RANKL Expression in Human Rheumatoid Arthritis Synovial Fibroblasts.PloS one · 2016Article
- Article
- Intracellular domain fragment of CD44 alters CD44 function in chondrocytes.The Journal of biological chemistry · 2013Article
- Injectable thermoreversible hyaluronan-based hydrogels for nucleus pulposus cell encapsulation.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2012Article
- Pathophysiological changes to the peritoneal membrane during PD-related peritonitis: the role of mesothelial cells.Mediators of inflammation · 2012Review
- Mechanisms involved in enhancement of the expression and function of aggrecanases by hyaluronan oligosaccharides.Arthritis and rheumatism · 2012Article
- Induction of CD44 cleavage in articular chondrocytes.Arthritis and rheumatism · 2010Article
- Hyaluronan oligosaccharide treatment of chondrocytes stimulates expression of both HAS-2 and MMP-3, but by different signaling pathways.Osteoarthritis and cartilage · 2010Article
- Hyaluronan promotes the chondrocyte response to BMP-7.Osteoarthritis and cartilage · 2009Article
- Role of sialidase in Mycoplasma alligatoris-induced pulmonary fibroblast apoptosis.Veterinary microbiology · 2007Article
- Expression of superficial zone protein in mandibular condyle cartilage.Osteoarthritis and cartilage · 2006Article
- Hyaluronan oligosaccharides induce matrix metalloproteinase 13 via transcriptional activation of NFkappaB and p38 MAP kinase in articular chondrocytes.The Journal of biological chemistry · 2006Article
- Hyaluronan fragments activate nitric oxide synthase and the production of nitric oxide by articular chondrocytes.The international journal of biochemistry & cell biology · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
objectiveTo document the activity profile of transcription factors following chondrocyte stimulation with hyaluronan (HA) hexasaccharides (HA(6)) and to determine the expression of genes whose transcriptional activation is tightly associated with the transcription factors.
methodsNuclear extracts from bovine articular chondrocytes treated with HA(6) were subjected to transcription factor protein-DNA array analysis. Electrophoretic mobility shift assay (EMSA) analyses were performed to confirm the results of protein-DNA array. The gene expressions of matrix metalloproteinase 3 (MMP-3), type II collagen, and cartilage oligomeric matrix protein (COMP) were examined by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR), and protease activity was assessed by casein zymography.
resultsIn the protein-DNA array analysis, 12 transcription factors were up-regulated and 2 transcription factors were down-regulated in the chondrocytes treated with HA(6). The transcription factors retinoic acid receptor (RAR), retinoid X receptor (RXR), and Sp-1 exhibited >2-fold increased activity by HA(6) treatment, as confirmed by EMSA. RT-PCR analysis showed that the expression levels of MMP-3, type II collagen, and COMP messenger RNA, which are tightly associated with the activation of RAR, RXR, or Sp-1, were up-regulated by treatment with HA(6). Addition of high molecular mass HA after HA(6) treatment resulted in abrogation of the MMP-3 induction.
conclusionThese results suggest that HA(6) increase the activity of multiple transcription factors in chondrocytes and signal the enhanced expression of key genes involved in cartilage-matrix remodeling and turnover. The data also demonstrate that high molecular mass HA has a potential to suppress the signaling activated by HA(6).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.