ArticleJournal of clinical microbiology2005
Multiplex real-time PCR assay using Scorpion probes and DNA capture for genotype-specific detection of Giardia lamblia on fecal samples.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Molecular Epidemiology of Giardia Species in Different Hosts in Africa: A Systematic Review and Meta-Analysis.Acta parasitologica · 2026Pooled it
- Association of Helicobacter Pylori in Chronic Tonsillitis.Indian journal of otolaryngology and head and neck surgery : official publication of the Association of Otolaryngologists of India · 2024Article
- Article
- The controversies surroundingCurrent research in parasitology & vector-borne diseases · 2021Review
- Scorpion primer PCR analysis for genotyping of allele variants of thiopurine s‑methyltransferase*3.Molecular medicine reports · 2020Article
- Stool antigen immunodetection for diagnosis of Giardia duodenalis infection in human subjects with HIV and cancer.Journal of microbiological methods · 2017Article
- Adaptive immune response in symptomatic and asymptomatic enteric protozoal infection: evidence for a determining role of parasite genetic heterogeneity in host immunity to human giardiasis.Microbes and infection · 2016Observational
- Association between stool enteropathogen quantity and disease in Tanzanian children using TaqMan array cards: a nested case-control study.The American journal of tropical medicine and hygiene · 2014Article
- Dual probe DNA capture for sensitive real-time PCR detection of Cryptosporidium and Giardia.Molecular and cellular probes · 2012Article
- Assessment of helicobacter pylori prevalence by scorpion real-time PCR in chronic tonsillitis patients.Journal of global infectious diseases · 2012Article
- High throughput multiplex PCR and probe-based detection with Luminex beads for seven intestinal parasites.The American journal of tropical medicine and hygiene · 2011Article
- Zoonotic potential and molecular epidemiology of Giardia species and giardiasis.Clinical microbiology reviews · 2011Review
- Vaccines to combat the neglected tropical diseases.Immunological reviews · 2011Review
- Giardia intestinalis assemblages A and B infections in Nepal.The American journal of tropical medicine and hygiene · 2009Article
- Current applications and future trends of molecular diagnostics in clinical bacteriology.Analytical and bioanalytical chemistry · 2009Review
- Prospective case-control study of the association between common enteric protozoal parasites and diarrhea in Bangladesh.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2009Article
- Evaluation of a screening test for detection of giardia and cryptosporidium parasites.Journal of clinical microbiology · 2009Article
- Giardia duodenalis assemblage, clinical presentation and markers of intestinal inflammation in Brazilian children.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2008Article
- Quadruplex real-time PCR assay using allele-specific scorpion primers for detection of mutations conferring clarithromycin resistance to Helicobacter pylori.Journal of clinical microbiology · 2008Article
- Human intestinal parasites.Journal of health, population, and nutrition · 2007Article
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Authors and funding
6 authors.
Funding
Abstract
Two major genotypic assemblages of Giardia lamblia infect humans; the epidemiologic significance of this phenomenon is poorly understood. We developed a single-vessel multiplex real-time PCR (qPCR) assay that genotypes Giardia infections into assemblages A and/or B directly from fecal samples. The assay utilized Scorpion probes that combined genotype-specific primers and probes for the 18S rRNA gene into the same molecule. The protocol was capable of detecting as few as 20 trophozoites per PCR on fecal DNA isolated using a commercial method or 1.25 trophozoites per PCR on fecal DNA isolated using a G. lamblia-specific oligonucleotide capture technique. The assay was specific for fecal specimens, with no amplification of the discordant genotype with the opposite Scorpion probe. When 97 clinical specimens from Bangladesh were used, the multiplex PCR assay detected 95% (21 of 22) of Giardia microscopy-positive specimens and 18% (13 of 74) of microscopy-negative specimens. Microscopy-negative and qPCR-positive specimens had higher average cycle threshold values than microscopy-positive and qPCR-positive specimens, suggesting that they represented true low-burden infections. Most (32 of 35) infections were assemblage B infections. This single-reaction multiplex qPCR assay distinguishes assemblage A Giardia infections from assemblage B infections directly on fecal samples and may aid epidemiologic investigation.
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