ArticleJournal of clinical microbiology2005
Potential limitations of the 16S-23S rRNA intergenic region for molecular detection of Bartonella species.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
72 citing papers in PubMed, 144 citations in OpenAlex.
- Molecular evidence of Bartonella spp. in wild felids from Brazil.Veterinary research communications · 2026Article
- Bartonella vinsonii subsp. berkhoffii genotype III in a dog with recurrent fever and panniculitis coinfected with Babesia odocoilei-like sp.Journal of veterinary internal medicine · 2026Article
- Bartonella transmission and gut microbiome dynamics in Ceratophyllus sciurorum fleas and their edible dormouse hosts (Glis glis).Parasites & vectors · 2026Article
- Molecular identification of bat fly species and associated Bartonella bacteria from Lopburi and Sa Kaeo Provinces in Thailand.Scientific reports · 2026Article
- An optimized 16S-23S rRNA intergenic spacer region PCR for the detection and identification ofMicrobiology spectrum · 2026Article
- Ectoparasite-borneParasitology · 2026Article
- First molecular evidence of Bartonella spp. and hemoplasmas in cattle from Mozambique.Tropical animal health and production · 2026Article
- Validation of a High-Throughput Microfluidic Real-Time PCR for the Detection of Vector-Borne Agents in Wild Birds from the Brazilian Pantanal.Pathogens (Basel, Switzerland) · 2025Article
- Exploring the Zoonotic Risk ofTransboundary and emerging diseases · 2025Article
- Diversity and Multiple Infections ofPathogens (Basel, Switzerland) · 2024Article
- Bartonella spp. in bats from the Brazilian Amazon Forest.Veterinary research communications · 2024Article
- Blood culture-negative endocarditis caused by Bartonella quintana in Iran.Scientific reports · 2024Article
- Prevalence and genetic diversity ofApplied and environmental microbiology · 2024Article
- Human Babesia odocoilei and Bartonella spp. co-infections in the Americas.Parasites & vectors · 2024Article
- Antibodies toMicrobiology spectrum · 2024Observational
- Comparison of molecular methods for Bartonella henselae detection in blood donors.PLoS neglected tropical diseases · 2023Article
- ProfilingJournal of veterinary science · 2023Article
- Molecular survey and phylogenetic analysis ofFrontiers in veterinary science · 2023Article
- Development of a quadruplex PCR amplicon next generation sequencing assay for detection and differentiation ofFrontiers in microbiology · 2023Article
- Combining deep sequencing and conventional molecular approaches reveals broad diversity and distribution of fleas and Bartonella in rodents and shrews from Arctic and Subarctic ecosystems.Parasites & vectors · 2022Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PCR targeting the 16S-23S rRNA gene intergenic transcribed spacer (ITS) region has been proposed as a rapid and reliable method for the detection of Bartonella species DNA in clinical samples. Because of variation in ITS sequences among Bartonella species, a single PCR amplification can be used to detect different species within this genus. Therefore, by targeting the ITS region, multiple PCRs or additional sample-processing steps beyond the primary amplification can be avoided when attempting to achieve molecular diagnostic detection of Bartonella species. Although PCR amplification targeting this region is considered highly sensitive, amplification specificity obviously depends on primer design. We report evidence of nonspecific PCR amplification of Mesorhizobium species with previously published primers that were designed to amplify the Bartonella consensus ITS region. Use of these or other, less species-specific, primers could lead to a false-positive diagnostic test result when evaluating clinical samples. We also report the presence of Mesorhizobium species DNA as a contaminant in molecular-grade water, a series of homologous sequences in the ITS region that are common to Bartonella and Mesorhizobium species, the amplification of Mesorhizobium DNA with unpublished primers designed in our laboratory targeting the ITS region, and the subsequent design of unambiguous ITS primers that avoid nonspecific amplification of Mesorhizobium species. Our results define some potential limitations associated with the molecular detection of Bartonella species in patient samples and indicate that primer specificity is of critical importance if the ITS region is used as a diagnostic target for detection of Bartonella species.
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