ArticleJournal of clinical microbiology2005
Use of diagnostic microarrays for determination of virulence gene patterns of Escherichia coli K1, a major cause of neonatal meningitis.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 58 citations in OpenAlex.
- Development and Assessment of a Diagnostic DNA Oligonucleotide Microarray for Detection and Typing of Meningitis-Associated Bacterial Species.High-throughput · 2018Article
- Virulence-associated genes and antibiotic susceptibility among vaginal and rectal Escherichia coli isolates from healthy pregnant women in Poland.Folia microbiologica · 2018Article
- Virulence genes and antimicrobial susceptibility of lactose-negative and lactose-positive strains of Escherichia coli isolated from pregnant women and neonates.Folia microbiologica · 2017Article
- Route of infection alters virulence of neonatal septicemia Escherichia coli clinical isolates.PloS one · 2017Article
- Simultaneous Detection of Five Pathogens from Cerebrospinal Fluid Specimens Using Luminex Technology.International journal of environmental research and public health · 2016Article
- Neonatal bacterial meningitis in Tikur Anbessa Specialized Hospital, Ethiopia: a 10-year retrospective review.SpringerPlus · 2016Article
- Detection of K1 antigen of Escherichia coli rods isolated from pregnant women and neonates.Folia microbiologica · 2014Article
- Pathotyping bla CTX-M Escherichia coli from Nigeria.European journal of microbiology & immunology · 2013Article
- Lethal inflammasome activation by a multidrug-resistant pathobiont upon antibiotic disruption of the microbiota.Nature medicine · 2012Article
- Characterisation of Australian MRSA strains ST75- and ST883-MRSA-IV and analysis of their accessory gene regulator locus.PloS one · 2010Article
- High-throughput microarray technology in diagnostics of enterobacteria based on genome-wide probe selection and regression analysis.BMC genomics · 2010Article
- Basic concepts of microarrays and potential applications in clinical microbiology.Clinical microbiology reviews · 2009Review
- Pathotyping Escherichia coli by using miniaturized DNA microarrays.Applied and environmental microbiology · 2007Article
- Fast DNA serotyping of Escherichia coli by use of an oligonucleotide microarray.Journal of clinical microbiology · 2007Article
- An alternative method to amplify RNA without loss of signal conservation for expression analysis with a proteinase DNA microarray in the ArrayTube format.BMC genomics · 2006Article
- Development and validation of an oligonucleotide microarray for detection of multiple virulence and antimicrobial resistance genes in Escherichia coli.Applied and environmental microbiology · 2006Article
- Use of miniaturized protein arrays for Escherichia coli O serotyping.Clinical and vaccine immunology : CVI · 2006Article
- Genomic comparison of Escherichia coli K1 strains isolated from the cerebrospinal fluid of patients with meningitis.Infection and immunity · 2006Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Forty Escherichia coli strains isolated primarily from neonatal meningitis, urinary tract infections and feces were screened for the presence of virulence genes with a newly developed microarray on the array tube format. A total of 32 gene probes specific for extraintestinal as well as intestinal E. coli pathotypes were included. Eighty-eight percent of the analyzed strains were positive for the K1-specific probe on the microarray and could be confirmed with a specific antiserum against the K1 capsular polysaccharide. The gene for the hemin receptor ChuA was predominantly found in 95% of strains. Other virulence genes associated with K1 and related strains were P, S, and F1C fimbriae specific for extraintestinal E. coli, the genes for aerobactin, the alpha-hemolysin and the cytotoxic necrotizing factor. In two strains, the O157-specific catalase gene and the gene for the low-molecular-weight heat-stable toxin AstA were detected, respectively. A total of 19 different virulence gene patterns were observed. No correlation was observed between specific virulence gene patterns and a clinical outcome. The data indicate that virulence genes typical of extraintestinal E. coli are predominantly present in K1 strains. Nevertheless, some of them can carry virulence genes known to be characteristic of intestinal E. coli. The distribution and combination of virulence genes show that K1 isolates constitute a heterogeneous group of E. coli.
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