ArticlePlant physiology2005
Proteomic analysis of somatic embryogenesis in Medicago truncatula. Explant cultures grown under 6-benzylaminopurine and 1-naphthaleneacetic acid treatments.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 130 citations in OpenAlex.
- The eTM-miR3699-MAN7 mediated cell wall degradation in regulating embryogenic cell formation during the early stage of somatic embryogenesis in apple.Horticulture research · 2026Article
- Transcriptional Dynamics Underlying Somatic Embryogenesis inPlants (Basel, Switzerland) · 2025Article
- Transcriptome and Gene Expression Analysis RevealedInternational journal of molecular sciences · 2024Article
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- Exogenous Glutathione Promotes the Proliferation ofPlants (Basel, Switzerland) · 2022Article
- Uncovering a Phenomenon of Active Hormone Transcriptional Regulation during Early Somatic Embryogenesis inInternational journal of molecular sciences · 2022Article
- Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production.Systems microbiology and biomanufacturing · 2022Article
- NaCl-responsive ROS scavenging and energy supply in alkaligrass callus revealed from proteomic analysis.BMC genomics · 2019Article
- Somatic Embryogenesis in theFrontiers in plant science · 2019Review
- Somatic Embryogenesis Induction in Woody Species: The Future After OMICs Data Assessment.Frontiers in plant science · 2019Review
- Proteomic analysis of the response of Funnelifor mismosseae/Medicago sativa to atrazine stress.BMC plant biology · 2018Article
- The Use of Proteomic Tools to Address Challenges Faced in Clonal Propagation of Tropical Crops through Somatic Embryogenesis.Proteomes · 2018Review
- Advanced Proteomic Approaches to Elucidate Somatic Embryogenesis.Frontiers in plant science · 2018Review
- Molecular Signals Controlling the Inhibition of Nodulation by Nitrate in Medicago truncatula.International journal of molecular sciences · 2016Article
- ROS Homeostasis Regulates Somatic Embryogenesis via the Regulation of Auxin Signaling in Cotton.Molecular & cellular proteomics : MCP · 2016Article
- De novo assembly and characterization of global transcriptome of coconut palm (Cocos nucifera L.) embryogenic calli using Illumina paired-end sequencing.Protoplasma · 2016Article
- Global scale transcriptome analysis of Arabidopsis embryogenesis in vitro.BMC genomics · 2015Article
- Article
- Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.Proteome science · 2014Article
- Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata.Journal of Zhejiang University. Science. B · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Medicago truncatula line 2HA has a 500-fold greater capacity to regenerate plants in culture by somatic embryogenesis than wild-type Jemalong. We have compared proteomes of tissue cultures from leaf explants of these two lines. Both 2HA and Jemalong explants were grown on media containing the auxin 1-naphthaleneacetic acid and the cytokinin 6-benzylaminopurine. Proteins were extracted from the cultures at different time points (2, 5, and 8 weeks), separated by two-dimensional gel electrophoresis, and detected by silver staining. More than 2,000 proteins could be reproducibly resolved and detected on each gel. Statistical analysis showed that 54 protein spots were significantly (P < 0.05) changed in expression (accumulation) during the 8 weeks of culture, and most of these spots were extracted from colloidal Coomassie-stained two-dimensional gel electrophoresis gels and were subjected to matrix-assisted laser desorption ionization time-of-flight mass spectrometry or liquid chromatography-tandem mass spectrometry analysis. Using a publicly available expressed sequence tag database and the Mascot search engine, we were able to identify 16 differentially expressed proteins. More than 60% of the differentially expressed protein spots had very different patterns of gene expression between 2HA and Jemalong during the 8 weeks of culture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.