ArticleBiophysical journal2005
Single-molecule analysis of epidermal growth factor signaling that leads to ultrasensitive calcium response.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 65 citations in OpenAlex.
- Counting fluorescently labeled proteins in tissues in the spinning-disk microscope using single-molecule calibrations.Molecular biology of the cell · 2022Article
- Characterizing Large-Scale Receptor Clustering on the Single Cell Level: A Comparative Plasmon Coupling and Fluorescence Superresolution Microscopy Study.The journal of physical chemistry. B · 2019Article
- Ligand-activated epidermal growth factor receptor (EGFR) signaling governs endocytic trafficking of unliganded receptor monomers by non-canonical phosphorylation.The Journal of biological chemistry · 2018Article
- Single-molecule analysis of cell surface dynamics in Caenorhabditis elegans embryos.Nature methods · 2014Article
- Simultaneous profiling of 194 distinct receptor transcripts in human cells.Science signaling · 2013Article
- A mathematical model for BRASSINOSTEROID INSENSITIVE1-mediated signaling in root growth and hypocotyl elongation.Plant physiology · 2012Article
- Dynamically varying interactions between heregulin and ErbB proteins detected by single-molecule analysis in living cells.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Physical-chemical principles underlying RTK activation, and their implications for human disease.Biochimica et biophysica acta · 2012Review
- Expression of excess receptors and negative feedback control of signal pathways are required for rapid activation and prompt cessation of signal transduction.Cell communication and signaling : CCS · 2009Article
- Modelling reaction kinetics inside cells.Essays in biochemistry · 2008Review
- How single molecule detection measures the dynamic actions of life.HFSP journal · 2007Article
- Compartment-specific feedback loop and regulated trafficking can result in sustained activation of Ras at the Golgi.Biophysical journal · 2007Article
- Single-molecule analysis of epidermal growth factor binding on the surface of living cells.The EMBO journal · 2006Article
- Imaging single molecules in living cells for systems biology.Molecular systems biology · 2006Review
- Role of network branching in eliciting differential short-term signaling responses in the hypersensitive epidermal growth factor receptor mutants implicated in lung cancer.Biotechnology progressArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative relationships between inputs and outputs of signaling systems are fundamental information for the understanding of the mechanism of signal transduction. Here we report the correlation between the number of epidermal growth factor (EGF) bindings and the response probability of intracellular calcium elevation. Binding of EGF molecules and changes of intracellular calcium concentration were measured for identical HeLa human epithelial cells. It was found that 300 molecules of EGF were enough to induce calcium response in half of the cells. This number is quite small compared to the number of EGF receptors (EGFR) expressed on the cell surface (50,000). There was a sigmoidal correlation between the response probability and the number of EGF bindings, meaning an ultrasensitive reaction. Analysis of the cluster size distribution of EGF demonstrated that dimerization of EGFR contributes to this switch-like ultrasensitive response. Single-molecule analysis revealed that EGF bound faster to clusters of EGFR than to monomers. This property should be important for effective formation of signaling dimers of EGFR under very small numbers of EGF bindings and suggests that the expression of excess amounts of EGFR on the cell surface is required to prepare predimers of EGFR with a large association rate constant to EGF.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.