ArticleBMC genomics2005
Global transcriptome analysis of the C57BL/6J mouse testis by SAGE: evidence for nonrandom gene order.
Article in BMC genomics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- A benchmark of gene expression tissue-specificity metrics.Briefings in bioinformatics · 2017Article
- Escape of X-linked miRNA genes from meiotic sex chromosome inactivation.Development (Cambridge, England) · 2015Article
- Article
- Expression analysis of MND1/GAJ, SPATA22, GAPDHS and ACR genes in testicular biopsies from non-obstructive azoospermia (NOA) patients.Reproductive biology and endocrinology : RB&E · 2013Article
- The computational analysis of human testis transcriptome reveals closer ties to pluripotency.Journal of human reproductive sciences · 2012Article
- Conserved properties of Drosophila and human spermatozoal mRNA repertoires.Proceedings. Biological sciences · 2012Article
- The role of Dby mRNA in early development of male mouse zygotes.Asian journal of andrology · 2010Article
- Testicular postgenomics: targeting the regulation of spermatogenesis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2010Review
- The chicken Z chromosome is enriched for genes with preferential expression in ovarian somatic cells.Journal of molecular evolution · 2010Article
- Review
- Recent origins of sperm genes in Drosophila.Molecular biology and evolution · 2008Article
- Structural conservation versus functional divergence of maternally expressed microRNAs in the Dlk1/Gtl2 imprinting region.BMC genomics · 2008Article
- Genetic and epigenetic mechanisms of gene regulation during lens development.Progress in retinal and eye research · 2007Review
- Nonrandom representation of sex-biased genes on chicken Z chromosome.Journal of molecular evolution · 2006Article
- A method for similarity search of genomic positional expression using CAGE.PLoS genetics · 2006Article
- Mating system and brain size in bats.Proceedings. Biological sciences · 2006Article
- Loss ofReproductive medicine and biologyArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWe generated the gene expression profile of the total testis from the adult C57BL/6J male mice using serial analysis of gene expression (SAGE). Two high-quality SAGE libraries containing a total of 76 854 tags were constructed. An extensive bioinformatic analysis and comparison of SAGE transcriptomes of the total testis, testicular somatic cells and other mouse tissues was performed and the theory of male-biased gene accumulation on the X chromosome was tested.
resultsWe sorted out 829 genes predominantly expressed from the germinal part and 944 genes from the somatic part of the testis. The genes preferentially and specifically expressed in total testis and testicular somatic cells were identified by comparing the testis SAGE transcriptomes to the available transcriptomes of seven non-testis tissues. We uncovered chromosomal clusters of adjacent genes with preferential expression in total testis and testicular somatic cells by a genome-wide search and found that the clusters encompassed a significantly higher number of genes than expected by chance. We observed a significant 3.2-fold enrichment of the proportion of X-linked genes specific for testicular somatic cells, while the proportions of X-linked genes specific for total testis and for other tissues were comparable. In contrast to the tissue-specific genes, an under-representation of X-linked genes in the total testis transcriptome but not in the transcriptomes of testicular somatic cells and other tissues was detected.
conclusionOur results provide new evidence in favor of the theory of male-biased genes accumulation on the X chromosome in testicular somatic cells and indicate the opposite action of the meiotic X-inactivation in testicular germ cells.
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