ArticleApplied and environmental microbiology2005
Quantitative-PCR assessment of Cryptosporidium parvum cell culture infection.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 59 citations in OpenAlex.
- Article
- Validation of a one-tube nested real-time PCR assay for the detection of Cryptosporidium spp. in avian fecal samples.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2022Article
- Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR.Parasites & vectors · 2019Article
- Fate of Manure-Borne Pathogens during Anaerobic Digestion and Solids Separation.Journal of environmental quality · 2018Article
- Assessing viability and infectivity of foodborne and waterborne stages (cysts/oocysts) of Giardia duodenalis, Cryptosporidium spp., and Toxoplasma gondii: a review of methods.Parasite (Paris, France) · 2018Review
- Hydrologic, land cover, and seasonal patterns of waterborne pathogens in Great Lakes tributaries.Water research · 2017Article
- Detection of hepatitis E virus and other livestock-related pathogens in Iowa streams.The Science of the total environment · 2016Article
- Public health significance of zoonoticInternational journal for parasitology. Parasites and wildlife · 2016Review
- Multicentric evaluation of a new real-time PCR assay for quantification of Cryptosporidium spp. and identification of Cryptosporidium parvum and Cryptosporidium hominis.Journal of clinical microbiology · 2013Article
- Article
- In vitro determination of anticryptosporidial activity of phytogenic extracts and compounds.Parasitology research · 2012Article
- Real-time PCR assay targeting the actin gene for the detection of Cryptosporidium parvum in calf fecal samples.Parasitology research · 2012Article
- Comparison of assays for sensitive and reproducible detection of cell culture-infectious Cryptosporidium parvum and Cryptosporidium hominis in drinking water.Applied and environmental microbiology · 2012Article
- Efficacy of ginkgolic acids against Cryptosporidium andersoni in cell culture.Parasitology research · 2011Article
- Detection and differentiation of Cryptosporidium spp. in human clinical samples by use of real-time PCR.Journal of clinical microbiology · 2011Article
- A screening pipeline for antiparasitic agents targeting cryptosporidium inosine monophosphate dehydrogenase.PLoS neglected tropical diseases · 2010Article
- Improved risk analysis by dual direct detection of total and infectious Cryptosporidium oocysts on cell culture in combination with immunofluorescence assay.Applied and environmental microbiology · 2010Article
- Cryptosporidium propidium monoazide-PCR, a molecular biology-based technique for genotyping of viable Cryptosporidium oocysts.Applied and environmental microbiology · 2009Article
- CP2 gene as a useful viability marker for Cryptosporidium parvum.Parasitology research · 2008Article
- Aged HCT-8 cell monolayers support Cryptosporidium parvum infection.Applied and environmental microbiology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A quantitative TaqMan PCR method was developed for assessing the Cryptosporidium parvum infection of in vitro cultivated human ileocecal adenocarcinoma (HCT-8) cell cultures. This method, termed cell culture quantitative sequence detection (CC-QSD), has numerous applications, several of which are presented. CC-QSD was used to investigate parasite infection in cell culture over time, the effects of oocyst treatment on infectivity and infectivity assessment of different C. parvum isolates. CC-QSD revealed that cell culture infection at 24 and 48 h postinoculation was approximately 20 and 60%, respectively, of the endpoint 72-h postinoculation infection. Evaluation of three different lots of C. parvum Iowa isolate oocysts revealed that the mean infection of 0.1 N HCl-treated oocysts was only 36% of the infection obtained with oocysts treated with acidified Hanks' balanced salt solution containing 1% trypsin. CC-QSD comparison of the C. parvum Iowa and TAMU isolates revealed significantly higher levels of infection for the TAMU isolate, which agrees with and supports previous human, animal, and cell culture studies. CC-QSD has the potential to aid in the optimization of Cryptosporidium cell culture methods and facilitate quantitative evaluation of cell culture infectivity experiments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.