ArticleApplied and environmental microbiology2005
Incidence and diversity of potentially highly heat-resistant spores isolated at dairy farms.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Dairy Spoilage Bacteria and Their Lipases: Prevalence Patterns and Molecular Traits.Foods (Basel, Switzerland) · 2026Article
- Isolation of Thermophilic Bacteria from Extreme Environments in Northern Chile.Microorganisms · 2024Article
- Characterization and spoilage potential ofFrontiers in microbiology · 2022Article
- Identification of Thermophilic Aerobic Sporeformers in Bedding Material of Compost-Bedded Dairy Cows Using Microbial and Molecular Methods.Animals : an open access journal from MDPI · 2021Article
- Molecular Physiological Characterization of a High Heat Resistant Spore FormingMicroorganisms · 2021Article
- Taxonomic Evaluation of theMicroorganisms · 2021Article
- Reservoirs of antimicrobial resistance genes in retail raw milk.Microbiome · 2020Article
- Genome Sequences of Bacillus sporothermodurans Strains Isolated from Ultra-High-Temperature Milk.Microbiology resource announcements · 2019Article
- Farm level survey of spore-forming bacteria on four dairy farms in the Waikato region of New Zealand.MicrobiologyOpen · 2017Article
- Draft genome sequence ofGenomics data · 2017Article
- Spore Heat Activation Requirements and Germination Responses Correlate with Sequences of Germinant Receptors and with the Presence of a SpecificApplied and environmental microbiology · 2017Article
- A mobile genetic element profoundly increases heat resistance of bacterial spores.The ISME journal · 2016Article
- Bacillus thermoamylovorans Spores with Very-High-Level Heat Resistance Germinate Poorly in Rich Medium despite the Presence of ger Clusters but Efficiently upon Exposure to Calcium-Dipicolinic Acid.Applied and environmental microbiology · 2015Article
- Draft Genome Sequences of Four Bacillus thermoamylovorans Strains Isolated from Milk and Acacia Gum, a Food Ingredient.Genome announcements · 2015Article
- The Prevalence and Control of Bacillus and Related Spore-Forming Bacteria in the Dairy Industry.Frontiers in microbiology · 2015Review
- Abiotic and microbiotic factors controlling biofilm formation by thermophilic sporeformers.Applied and environmental microbiology · 2013Article
- Microbiota dynamics and diversity at different stages of industrial processing of cocoa beans into cocoa powder.Applied and environmental microbiology · 2012Article
- Bacterial diversity and structural changes of oyster shell during 1-year storage.Microbial ecology · 2009Article
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5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The presence of highly heat-resistant spores of Bacillus sporothermodurans in ultrahigh-temperature or sterilized consumer milk has emerged as an important item in the dairy industry. Their presence is considered undesirable since they hamper the achievement of commercial sterility requirements. By using a selective 30-min heat treatment at 100 degrees C, 17 Belgian dairy farms were screened to evaluate the presence, sources, and nature of potentially highly heat-resistant spores in raw milk. High numbers of these spores were detected in the filter cloth of the milking equipment and in green crop and fodder samples. About 700 strains were isolated after the selective heating, of which 635 could be screened by fatty acid methyl ester analysis. Representative strains were subjected to amplified ribosomal DNA restriction analysis, 16S rRNA gene sequencing, percent G+C content, and DNA-DNA reassociations for further identification. The strain collection showed a remarkable diversity, with representatives of seven aerobic spore-forming genera. Bacillus licheniformis and Bacillus pallidus were the most predominant species overall. Twenty-three percent of the 603 spore-forming isolates proved to belong to 18 separate novel species. These findings suggest that the selective heating revealed a pool of unknown organisms with a higher heat-resistant character. This study showed that high spore counts can occur at the dairy farm and that feed and milking equipment can act as reservoirs or entry points for potentially highly heat-resistant spores into raw milk. Lowering this spore load by good hygienic measures could probably further reduce the contamination level of raw milk, in this way minimizing the aerobic spore-forming bacteria that could lead to spoilage of milk and dairy products. Assessment and characterization of this particular flora are of great importance to allow the dairy or food industry to adequately deal with newly arising microbiological problems.
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