ArticleApplied and environmental microbiology2005
16S rRNA gene-based oligonucleotide microarray for environmental monitoring of the betaproteobacterial order "Rhodocyclales".
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed.
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- On the diversity, phylogeny and biogeography of cable bacteria.Frontiers in microbiology · 2024Article
- Blind spots of universal primers and specific FISH probes for functional microbe and community characterization in EBPR systems.ISME communications · 2024Article
- Understanding the Variation of Bacteria in Response to Summertime Oxygen Depletion in Water Column of Bohai Sea.Frontiers in microbiology · 2022Article
- Article
- Phylogenomics of Rhodocyclales and its distribution in wastewater treatment systems.Scientific reports · 2020Article
- In situ identification of polyhydroxyalkanoate (PHA)-accumulating microorganisms in mixed microbial cultures under feast/famine conditions.Scientific reports · 2020Article
- Biofilm Formation Plays a Crucial Rule in the Initial Step of Carbon Steel Corrosion in Air and Water Environments.Materials (Basel, Switzerland) · 2020Article
- A Plant Growth-Promoting Microbial Soil Amendment Dynamically Alters the Strawberry Root Bacterial Microbiome.Scientific reports · 2019Article
- Thiotrophic bacterial symbiont induces polyphenism in giant ciliate host Zoothamnium niveum.Scientific reports · 2019Article
- Diversity and Co-Occurrence Patterns of Soil Bacterial and Fungal Communities of ChineseMicroorganisms · 2019Article
- Transmating: conjugative transfer of a new broad host range expression vector to various Bacillus species using a single protocol.BMC microbiology · 2018Article
- Analysis of Bacterial Community Structure of Activated Sludge from Wastewater Treatment Plants in Winter.BioMed research international · 2018Article
- Short-term microbial effects of a large-scale mine-tailing storage facility collapse on the local natural environment.PloS one · 2018Article
- Microbial community compositions in different functional zones of Carrousel oxidation ditch system for domestic wastewater treatment.AMB Express · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For simultaneous identification of members of the betaproteobacterial order "Rhodocyclales" in environmental samples, a 16S rRNA gene-targeted oligonucleotide microarray (RHC-PhyloChip) consisting of 79 probes was developed. Probe design was based on phylogenetic analysis of available 16S rRNA sequences from all cultured and as yet uncultured members of the "Rhodocyclales." The multiple nested probe set was evaluated for microarray hybridization with 16S rRNA gene PCR amplicons from 29 reference organisms. Subsequently, the RHC-PhyloChip was successfully used for cultivation-independent "Rhodocyclales" diversity analysis in activated sludge from an industrial wastewater treatment plant. The implementation of a newly designed "Rhodocyclales"-selective PCR amplification system prior to microarray hybridization greatly enhanced the sensitivity of the RHC-PhyloChip and thus enabled the detection of "Rhodocyclales" populations with relative abundances of less than 1% of all bacteria (as determined by fluorescence in situ hybridization) in the activated sludge. The presence of as yet uncultured Zoogloea-, Ferribacterium/Dechloromonas-, and Sterolibacterium-related bacteria in the industrial activated sludge, as indicated by the RHC-PhyloChip analysis, was confirmed by retrieval of their 16S rRNA gene sequences and subsequent phylogenetic analysis, demonstrating the suitability of the RHC-PhyloChip as a novel monitoring tool for environmental microbiology.
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